TY - BOOK
T1 - Non-Coding RNAs in Arabidopsis
T2 - Discovery of Nuclear ncRNAs from Body Methylated Genes and of miRNA Pathway Inhibition by Conserved, Bacterial Virulence Factor
AU - van Wonterghem, Miranda
PY - 2015
Y1 - 2015
N2 - This work evolves around elucidating the mechanisms of micro RNAs (miRNAs) in Arabidopsis thaliana. I identified a new class of nuclear non-coding RNAs derived from protein coding genes. The genes are miRNA targets with extensive gene body methylation. The RNA species are nuclear localized and despite lacking caps and poly-adenylation they are stable, which suggests that they are functional. In a second project I discovered that a conserved bacterial effector protein (HopX1) interacts with, and possibly degrades, a plant protein (MAD6) required for miRNA-guided repression of translation. The induction of the bacterial effector in plants results in defects in the miRNA activity comparable to the mutation of the MAD6 gene. Thus, the effector enables the bacterium to manipulate the essential miRNA pathway of the plant host
AB - This work evolves around elucidating the mechanisms of micro RNAs (miRNAs) in Arabidopsis thaliana. I identified a new class of nuclear non-coding RNAs derived from protein coding genes. The genes are miRNA targets with extensive gene body methylation. The RNA species are nuclear localized and despite lacking caps and poly-adenylation they are stable, which suggests that they are functional. In a second project I discovered that a conserved bacterial effector protein (HopX1) interacts with, and possibly degrades, a plant protein (MAD6) required for miRNA-guided repression of translation. The induction of the bacterial effector in plants results in defects in the miRNA activity comparable to the mutation of the MAD6 gene. Thus, the effector enables the bacterium to manipulate the essential miRNA pathway of the plant host
UR - https://rex.kb.dk/primo-explore/fulldisplay?docid=KGL01009175713&context=L&vid=NUI&search_scope=KGL&tab=default_tab&lang=da_DK
M3 - Ph.D. thesis
BT - Non-Coding RNAs in Arabidopsis
PB - Department of Biology, Faculty of Science, University of Copenhagen
ER -