No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions

Petrine Wellendorph; Lars Dan Johansen; Anders Asbjørn Jensen; Emilio Casanova; Martin Gassmann; Pierre Deprez; Philippe Clément-Lacroix; Bernhard Bettler; Hans Bräuner-Osborne

doi:10.1677/JME-08-0149

No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions

Petrine Wellendorph, Lars Dan Johansen, Anders Asbjørn Jensen, Emilio Casanova, Martin Gassmann, Pierre Deprez, Philippe Clément-Lacroix, Bernhard Bettler, Hans Bräuner-Osborne

58 Citationer (Scopus)

Abstract

GPRC6A is a seven transmembrane receptor mediating signaling by a wide range of L-alpha-amino-acids, a signaling augmented by the divalent cations Ca2+ and Mg2+. GPRC6A transcripts are detected in numerous mammalian tissues, but the physiological role of the receptor is thus far elusive. Analogously to the closely related calcium-sensing receptor, GPRC6A has been proposed to function as a metabolic sensor of Ca2+ and amino acids in bone and other tissues. In the present study, we have generated the first GPRC6A knockout mice and studied their phenotype with particular focus on bone homeostasis. The generated GPRC6A knockout mice are viable and fertile, develop normally and exhibit no significant differences in body weight compared to wild type littermates. Assessment of bone mineral density, histomorphometry and bone metabolism demonstrated no significant differences between 13-week-old knockout and wild type mice. In conclusion, our data do not support a role for GPRC6A in normal bone physiology.

Originalsprog	Engelsk
Tidsskrift	Journal of Molecular Endocrinology
Vol/bind	42
Udgave nummer	3
Sider (fra-til)	215-223
ISSN	0952-5041
DOI	https://doi.org/10.1677/JME-08-0149
Status	Udgivet - 2009

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title = "No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions",

abstract = "GPRC6A is a seven transmembrane receptor mediating signaling by a wide range of L-alpha-amino-acids, a signaling augmented by the divalent cations Ca2+ and Mg2+. GPRC6A transcripts are detected in numerous mammalian tissues, but the physiological role of the receptor is thus far elusive. Analogously to the closely related calcium-sensing receptor, GPRC6A has been proposed to function as a metabolic sensor of Ca2+ and amino acids in bone and other tissues. In the present study, we have generated the first GPRC6A knockout mice and studied their phenotype with particular focus on bone homeostasis. The generated GPRC6A knockout mice are viable and fertile, develop normally and exhibit no significant differences in body weight compared to wild type littermates. Assessment of bone mineral density, histomorphometry and bone metabolism demonstrated no significant differences between 13-week-old knockout and wild type mice. In conclusion, our data do not support a role for GPRC6A in normal bone physiology.",

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author = "Petrine Wellendorph and Johansen, {Lars Dan} and Jensen, {Anders Asbj{\o}rn} and Emilio Casanova and Martin Gassmann and Pierre Deprez and Philippe Cl{\'e}ment-Lacroix and Bernhard Bettler and Hans Br{\"a}uner-Osborne",

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T1 - No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions

AU - Wellendorph, Petrine

AU - Johansen, Lars Dan

AU - Jensen, Anders Asbjørn

AU - Casanova, Emilio

AU - Gassmann, Martin

AU - Deprez, Pierre

AU - Clément-Lacroix, Philippe

AU - Bettler, Bernhard

AU - Bräuner-Osborne, Hans

PY - 2009

Y1 - 2009

N2 - GPRC6A is a seven transmembrane receptor mediating signaling by a wide range of L-alpha-amino-acids, a signaling augmented by the divalent cations Ca2+ and Mg2+. GPRC6A transcripts are detected in numerous mammalian tissues, but the physiological role of the receptor is thus far elusive. Analogously to the closely related calcium-sensing receptor, GPRC6A has been proposed to function as a metabolic sensor of Ca2+ and amino acids in bone and other tissues. In the present study, we have generated the first GPRC6A knockout mice and studied their phenotype with particular focus on bone homeostasis. The generated GPRC6A knockout mice are viable and fertile, develop normally and exhibit no significant differences in body weight compared to wild type littermates. Assessment of bone mineral density, histomorphometry and bone metabolism demonstrated no significant differences between 13-week-old knockout and wild type mice. In conclusion, our data do not support a role for GPRC6A in normal bone physiology.

AB - GPRC6A is a seven transmembrane receptor mediating signaling by a wide range of L-alpha-amino-acids, a signaling augmented by the divalent cations Ca2+ and Mg2+. GPRC6A transcripts are detected in numerous mammalian tissues, but the physiological role of the receptor is thus far elusive. Analogously to the closely related calcium-sensing receptor, GPRC6A has been proposed to function as a metabolic sensor of Ca2+ and amino acids in bone and other tissues. In the present study, we have generated the first GPRC6A knockout mice and studied their phenotype with particular focus on bone homeostasis. The generated GPRC6A knockout mice are viable and fertile, develop normally and exhibit no significant differences in body weight compared to wild type littermates. Assessment of bone mineral density, histomorphometry and bone metabolism demonstrated no significant differences between 13-week-old knockout and wild type mice. In conclusion, our data do not support a role for GPRC6A in normal bone physiology.

KW - Former Faculty of Pharmaceutical Sciences

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DO - 10.1677/JME-08-0149

M3 - Journal article

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SN - 0952-5041

VL - 42

SP - 215

EP - 223

JO - Journal of Molecular Endocrinology

JF - Journal of Molecular Endocrinology

IS - 3

ER -

No evidence for a bone phenotype in GPRC6A knockout mice under normal physiological conditions

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