No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts

TY - JOUR

T1 - No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts

AU - Bayer, Monika L

AU - Schjerling, Peter

AU - Biskup, Edyta

AU - Herchenhan, Andreas

AU - Heinemeier, Katja M

AU - Doessing, Simon

AU - Krogsgaard, Michael

AU - Kjær, Michael

N1 - Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

PY - 2012/5

Y1 - 2012/5

N2 - The aging process of tendon tissue is associated with decreased collagen content and increased risk for injuries. An essential factor in tendon physiology is transforming growth factor-β1 (TGF-β1), which is presumed to be reduced systemically with advanced age. The aim of this study was to investigate whether human serum from elderly donors would have an inhibiting effect on the expression of collagen and collagen-related genes as well as on cell proliferative capacity in tendon cells from young individuals. There was no difference in systemic TGF-β1 levels in serum obtained from young and elderly donors, and we found no difference in collagen expression when cells were subjected to human serum from elderly versus young donors. In addition, tendon cell proliferation was similar when culture medium was supplemented with serum of different donor age. These findings suggest that factors such as the cell intrinsic capacity or the tissue-specific environment rather than systemic circulating factors are important for functional capacity throughout life in human tendon cells.

AB - The aging process of tendon tissue is associated with decreased collagen content and increased risk for injuries. An essential factor in tendon physiology is transforming growth factor-β1 (TGF-β1), which is presumed to be reduced systemically with advanced age. The aim of this study was to investigate whether human serum from elderly donors would have an inhibiting effect on the expression of collagen and collagen-related genes as well as on cell proliferative capacity in tendon cells from young individuals. There was no difference in systemic TGF-β1 levels in serum obtained from young and elderly donors, and we found no difference in collagen expression when cells were subjected to human serum from elderly versus young donors. In addition, tendon cell proliferation was similar when culture medium was supplemented with serum of different donor age. These findings suggest that factors such as the cell intrinsic capacity or the tissue-specific environment rather than systemic circulating factors are important for functional capacity throughout life in human tendon cells.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aging

KW - Cell Proliferation

KW - Cells, Cultured

KW - Collagen

KW - Fibroblasts

KW - Humans

KW - Male

KW - Middle Aged

KW - Serum

KW - Signal Transduction

KW - Tendons

KW - Transforming Growth Factor beta1

KW - Young Adult

U2 - 10.1016/j.mad.2012.02.002

DO - 10.1016/j.mad.2012.02.002

M3 - Journal article

C2 - 22395123

SN - 0047-6374

VL - 133

SP - 246

EP - 254

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

IS - 5

ER -