Kolpen, M., Kühl, M., Bjarnsholt, T., Moser, C. E., Hansen, C. R., Liengaard, L., Kharazmi, A., Pressler, T., Høiby, N., & Jensen, P. Ø. (2014). Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection. PLOS ONE, 9(1), e84353. https://doi.org/10.1371/journal.pone.0084353
Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection. /
Kolpen, Mette; Kühl, Michael; Bjarnsholt, Thomas et al.
I:
PLOS ONE, Bind 9, Nr. 1, 17.01.2014, s. e84353.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Kolpen, M, Kühl, M, Bjarnsholt, T, Moser, CE, Hansen, CR, Liengaard, L, Kharazmi, A, Pressler, T, Høiby, N & Jensen, PØ 2014, 'Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection', PLOS ONE, bind 9, nr. 1, s. e84353. https://doi.org/10.1371/journal.pone.0084353
Kolpen M, Kühl M, Bjarnsholt T, Moser CE, Hansen CR, Liengaard L et al. Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection. PLOS ONE. 2014 jan. 17;9(1):e84353. doi: 10.1371/journal.pone.0084353
Kolpen, Mette ; Kühl, Michael ; Bjarnsholt, Thomas et al. / Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection. I: PLOS ONE. 2014 ; Bind 9, Nr. 1. s. e84353.
@article{57d4e8585a814e869bb509f6624a29ba,
title = "Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection",
abstract = "Chronic lung infection by Pseudomonas aeruginosa is the major severe complication in cystic fibrosis (CF) patients, where P. aeruginosa persists and grows in biofilms in the endobronchial mucus under hypoxic conditions. Numerous polymorphonuclear leukocytes (PMNs) surround the biofilms and create local anoxia by consuming the majority of O2 for production of reactive oxygen species (ROS). We hypothesized that P. aeruginosa acquires energy for growth in anaerobic endobronchial mucus by denitrification, which can be demonstrated by production of nitrous oxide (N2O), an intermediate in the denitrification pathway. We measured N2O and O2 with electrochemical microsensors in 8 freshly expectorated sputum samples from 7 CF patients with chronic P. aeruginosa infection. The concentrations of NO3(-) and NO2(-) in sputum were estimated by the Griess reagent. We found a maximum median concentration of 41.8 µM N2O (range 1.4-157.9 µM N2O). The concentration of N2O in the sputum was higher below the oxygenated layers. In 4 samples the N2O concentration increased during the initial 6 h of measurements before decreasing for approximately 6 h. Concomitantly, the concentration of NO3(-) decreased in sputum during 24 hours of incubation. We demonstrate for the first time production of N2O in clinical material from infected human airways indicating pathogenic metabolism based on denitrification. Therefore, P. aeruginosa may acquire energy for growth by denitrification in anoxic endobronchial mucus in CF patients. Such ability for anaerobic growth may be a hitherto ignored key aspect of chronic P. aeruginosa infections that can inform new strategies for treatment and prevention.",
author = "Mette Kolpen and Michael K{\"u}hl and Thomas Bjarnsholt and Moser, {Claus Ernst} and Hansen, {Christine R{\o}nne} and Lars Liengaard and Arsalan Kharazmi and Tanja Pressler and Niels H{\o}iby and Jensen, {Peter {\O}strup}",
year = "2014",
month = jan,
day = "17",
doi = "10.1371/journal.pone.0084353",
language = "English",
volume = "9",
pages = "e84353",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",
}
TY - JOUR
T1 - Nitrous oxide production in sputum from cystic fibrosis patients with chronic Pseudomonas aeruginosa lung infection
AU - Kolpen, Mette
AU - Kühl, Michael
AU - Bjarnsholt, Thomas
AU - Moser, Claus Ernst
AU - Hansen, Christine Rønne
AU - Liengaard, Lars
AU - Kharazmi, Arsalan
AU - Pressler, Tanja
AU - Høiby, Niels
AU - Jensen, Peter Østrup
PY - 2014/1/17
Y1 - 2014/1/17
N2 - Chronic lung infection by Pseudomonas aeruginosa is the major severe complication in cystic fibrosis (CF) patients, where P. aeruginosa persists and grows in biofilms in the endobronchial mucus under hypoxic conditions. Numerous polymorphonuclear leukocytes (PMNs) surround the biofilms and create local anoxia by consuming the majority of O2 for production of reactive oxygen species (ROS). We hypothesized that P. aeruginosa acquires energy for growth in anaerobic endobronchial mucus by denitrification, which can be demonstrated by production of nitrous oxide (N2O), an intermediate in the denitrification pathway. We measured N2O and O2 with electrochemical microsensors in 8 freshly expectorated sputum samples from 7 CF patients with chronic P. aeruginosa infection. The concentrations of NO3(-) and NO2(-) in sputum were estimated by the Griess reagent. We found a maximum median concentration of 41.8 µM N2O (range 1.4-157.9 µM N2O). The concentration of N2O in the sputum was higher below the oxygenated layers. In 4 samples the N2O concentration increased during the initial 6 h of measurements before decreasing for approximately 6 h. Concomitantly, the concentration of NO3(-) decreased in sputum during 24 hours of incubation. We demonstrate for the first time production of N2O in clinical material from infected human airways indicating pathogenic metabolism based on denitrification. Therefore, P. aeruginosa may acquire energy for growth by denitrification in anoxic endobronchial mucus in CF patients. Such ability for anaerobic growth may be a hitherto ignored key aspect of chronic P. aeruginosa infections that can inform new strategies for treatment and prevention.
AB - Chronic lung infection by Pseudomonas aeruginosa is the major severe complication in cystic fibrosis (CF) patients, where P. aeruginosa persists and grows in biofilms in the endobronchial mucus under hypoxic conditions. Numerous polymorphonuclear leukocytes (PMNs) surround the biofilms and create local anoxia by consuming the majority of O2 for production of reactive oxygen species (ROS). We hypothesized that P. aeruginosa acquires energy for growth in anaerobic endobronchial mucus by denitrification, which can be demonstrated by production of nitrous oxide (N2O), an intermediate in the denitrification pathway. We measured N2O and O2 with electrochemical microsensors in 8 freshly expectorated sputum samples from 7 CF patients with chronic P. aeruginosa infection. The concentrations of NO3(-) and NO2(-) in sputum were estimated by the Griess reagent. We found a maximum median concentration of 41.8 µM N2O (range 1.4-157.9 µM N2O). The concentration of N2O in the sputum was higher below the oxygenated layers. In 4 samples the N2O concentration increased during the initial 6 h of measurements before decreasing for approximately 6 h. Concomitantly, the concentration of NO3(-) decreased in sputum during 24 hours of incubation. We demonstrate for the first time production of N2O in clinical material from infected human airways indicating pathogenic metabolism based on denitrification. Therefore, P. aeruginosa may acquire energy for growth by denitrification in anoxic endobronchial mucus in CF patients. Such ability for anaerobic growth may be a hitherto ignored key aspect of chronic P. aeruginosa infections that can inform new strategies for treatment and prevention.
U2 - 10.1371/journal.pone.0084353
DO - 10.1371/journal.pone.0084353
M3 - Journal article
C2 - 24465406
SN - 1932-6203
VL - 9
SP - e84353
JO - PLOS ONE
JF - PLOS ONE
IS - 1
ER -
