New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria

TY - JOUR

T1 - New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria

AU - Andersen, J B

AU - Sternberg, C

AU - Poulsen, L K

AU - Bjorn, S P

AU - Givskov, M

AU - Molin, Søren

PY - 1998

Y1 - 1998

N2 - Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.

AB - Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.

M3 - Journal article

C2 - 9603842

SN - 0099-2240

VL - 64

SP - 2240

EP - 2246

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 6

ER -