Neuronal damage by secretory phospholipase A2: modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture

Miriam Kolko; Elena B Rodriguez de Turco; Nils H Diemer; Nicolas G Bazan; Miriam Kolko

Neuronal damage by secretory phospholipase A2: modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture

Miriam Kolko, Elena B Rodriguez de Turco, Nils H Diemer, Nicolas G Bazan, Miriam Kolko

37 Citationer (Scopus)

Abstract

Activation of cytosolic phospholipase A(2) (cPLA(2)) is an early event in brain injury, which leads to the formation and accumulation of bioactive lipids: platelet-activating factor (PAF), free arachidonic acid, and eicosanoids. A cross-talk between secretory PLA(2) (sPLA(2)) and cPLA(2) in neural signal transduction has previously been suggested (J Biol Chem 271:32722; 1996). Here we show, using neuronal cell cultures, an up-regulation of cPLA(2) expression and an inhibition by the selective cPLA(2) inhibitor AACOCF3 after exposure to neurotoxic concentrations of sPLA(2)-OS2. Pretreatment of neuronal cultures with recombinant PAF acetylhydrolase (rPAF-AH) or the presynaptic PAF receptor antagonist, BN52021, partially blocked neuronal cell death induced by sPLA(2)-OS2. Furthermore, selective COX-2 inhibitors ameliorated sPLA(2)-OS2-induced neurotoxicity. We conclude that sPLA(2)-OS2 activates a neuronal signaling cascade that includes activation of cPLA(2), arachidonic acid release, PAF production, and induction of COX-2.

Originalsprog	Engelsk
Tidsskrift	Neuroscience Letters
Vol/bind	338
Udgave nummer	2
Sider (fra-til)	164-8
Antal sider	5
ISSN	0304-3940
Status	Udgivet - 27 feb. 2003

Citationsformater

Neuronal damage by secretory phospholipase A2: modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture. / Kolko, Miriam; Rodriguez de Turco, Elena B; Diemer, Nils H et al.
I: Neuroscience Letters, Bind 338, Nr. 2, 27.02.2003, s. 164-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Neuronal damage by secretory phospholipase A2: modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture",

abstract = "Activation of cytosolic phospholipase A(2) (cPLA(2)) is an early event in brain injury, which leads to the formation and accumulation of bioactive lipids: platelet-activating factor (PAF), free arachidonic acid, and eicosanoids. A cross-talk between secretory PLA(2) (sPLA(2)) and cPLA(2) in neural signal transduction has previously been suggested (J Biol Chem 271:32722; 1996). Here we show, using neuronal cell cultures, an up-regulation of cPLA(2) expression and an inhibition by the selective cPLA(2) inhibitor AACOCF3 after exposure to neurotoxic concentrations of sPLA(2)-OS2. Pretreatment of neuronal cultures with recombinant PAF acetylhydrolase (rPAF-AH) or the presynaptic PAF receptor antagonist, BN52021, partially blocked neuronal cell death induced by sPLA(2)-OS2. Furthermore, selective COX-2 inhibitors ameliorated sPLA(2)-OS2-induced neurotoxicity. We conclude that sPLA(2)-OS2 activates a neuronal signaling cascade that includes activation of cPLA(2), arachidonic acid release, PAF production, and induction of COX-2.",

keywords = "1-Alkyl-2-acetylglycerophosphocholine Esterase, Animals, Arachidonic Acids, Cell Death, Cells, Cultured, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors, Cyclooxygenase Inhibitors, Cytosol, Diterpenes, Embryo, Mammalian, Enzyme Induction, Ginkgolides, Isoenzymes, L-Lactate Dehydrogenase, Lactones, Neurons, Neurotoxins, Phospholipases A, Phospholipases A2, Platelet Activating Factor, Platelet Membrane Glycoproteins, Prostaglandin-Endoperoxide Synthases, Rats, Receptors, Cell Surface, Receptors, G-Protein-Coupled, Recombinant Proteins, Reptilian Proteins, Up-Regulation",

author = "Miriam Kolko and {Rodriguez de Turco}, {Elena B} and Diemer, {Nils H} and Bazan, {Nicolas G} and Miriam Kolko",

year = "2003",

month = feb,

day = "27",

language = "English",

volume = "338",

pages = "164--8",

journal = "Neuroscience Letters",

issn = "0304-3940",

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TY - JOUR

T1 - Neuronal damage by secretory phospholipase A2

T2 - modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture

AU - Kolko, Miriam

AU - Rodriguez de Turco, Elena B

AU - Diemer, Nils H

AU - Bazan, Nicolas G

AU - Kolko, Miriam

PY - 2003/2/27

Y1 - 2003/2/27

N2 - Activation of cytosolic phospholipase A(2) (cPLA(2)) is an early event in brain injury, which leads to the formation and accumulation of bioactive lipids: platelet-activating factor (PAF), free arachidonic acid, and eicosanoids. A cross-talk between secretory PLA(2) (sPLA(2)) and cPLA(2) in neural signal transduction has previously been suggested (J Biol Chem 271:32722; 1996). Here we show, using neuronal cell cultures, an up-regulation of cPLA(2) expression and an inhibition by the selective cPLA(2) inhibitor AACOCF3 after exposure to neurotoxic concentrations of sPLA(2)-OS2. Pretreatment of neuronal cultures with recombinant PAF acetylhydrolase (rPAF-AH) or the presynaptic PAF receptor antagonist, BN52021, partially blocked neuronal cell death induced by sPLA(2)-OS2. Furthermore, selective COX-2 inhibitors ameliorated sPLA(2)-OS2-induced neurotoxicity. We conclude that sPLA(2)-OS2 activates a neuronal signaling cascade that includes activation of cPLA(2), arachidonic acid release, PAF production, and induction of COX-2.

AB - Activation of cytosolic phospholipase A(2) (cPLA(2)) is an early event in brain injury, which leads to the formation and accumulation of bioactive lipids: platelet-activating factor (PAF), free arachidonic acid, and eicosanoids. A cross-talk between secretory PLA(2) (sPLA(2)) and cPLA(2) in neural signal transduction has previously been suggested (J Biol Chem 271:32722; 1996). Here we show, using neuronal cell cultures, an up-regulation of cPLA(2) expression and an inhibition by the selective cPLA(2) inhibitor AACOCF3 after exposure to neurotoxic concentrations of sPLA(2)-OS2. Pretreatment of neuronal cultures with recombinant PAF acetylhydrolase (rPAF-AH) or the presynaptic PAF receptor antagonist, BN52021, partially blocked neuronal cell death induced by sPLA(2)-OS2. Furthermore, selective COX-2 inhibitors ameliorated sPLA(2)-OS2-induced neurotoxicity. We conclude that sPLA(2)-OS2 activates a neuronal signaling cascade that includes activation of cPLA(2), arachidonic acid release, PAF production, and induction of COX-2.

KW - 1-Alkyl-2-acetylglycerophosphocholine Esterase

KW - Animals

KW - Arachidonic Acids

KW - Cell Death

KW - Cells, Cultured

KW - Cyclooxygenase 2

KW - Cyclooxygenase 2 Inhibitors

KW - Cyclooxygenase Inhibitors

KW - Cytosol

KW - Diterpenes

KW - Embryo, Mammalian

KW - Enzyme Induction

KW - Ginkgolides

KW - Isoenzymes

KW - L-Lactate Dehydrogenase

KW - Lactones

KW - Neurons

KW - Neurotoxins

KW - Phospholipases A

KW - Phospholipases A2

KW - Platelet Activating Factor

KW - Platelet Membrane Glycoproteins

KW - Prostaglandin-Endoperoxide Synthases

KW - Rats

KW - Receptors, Cell Surface

KW - Receptors, G-Protein-Coupled

KW - Recombinant Proteins

KW - Reptilian Proteins

KW - Up-Regulation

M3 - Journal article

C2 - 12566178

SN - 0304-3940

VL - 338

SP - 164

EP - 168

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2

ER -

Neuronal damage by secretory phospholipase A2: modulation by cytosolic phospholipase A2, platelet-activating factor, and cyclooxygenase-2 in neuronal cells in culture

Abstract

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