TY - JOUR
T1 - Nedd8 processing enzymes in Schizosaccharomyces pombe
AU - O'Donoghue, Jean
AU - Bech-Otschir, Dawadschargal
AU - Larsen, Ida
AU - Wallace, Mairi
AU - Hartmann-Petersen, Rasmus
AU - Gordon, Colin
PY - 2013
Y1 - 2013
N2 - Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1. Results: Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo. Conclusions: Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.
AB - Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1. Results: Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo. Conclusions: Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.
U2 - 10.1186/1471-2091-14-8
DO - 10.1186/1471-2091-14-8
M3 - Journal article
C2 - 23496905
SN - 1471-2091
VL - 14
SP - 1
EP - 6
JO - B M C Biochemistry
JF - B M C Biochemistry
IS - 8
ER -