Nedd8 processing enzymes in Schizosaccharomyces pombe

Jean O'Donoghue; Dawadschargal Bech-Otschir; Ida Larsen; Mairi Wallace; Rasmus Hartmann-Petersen; Colin Gordon

doi:10.1186/1471-2091-14-8

Nedd8 processing enzymes in Schizosaccharomyces pombe

Jean O'Donoghue, Dawadschargal Bech-Otschir, Ida Larsen, Mairi Wallace, Rasmus Hartmann-Petersen, Colin Gordon

Biomolecular Sciences

5 Citationer (Scopus)

446 Downloads (Pure)

Abstract

Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1.

Originalsprog	Engelsk
Tidsskrift	B M C Biochemistry
Vol/bind	14
Udgave nummer	8
Sider (fra-til)	1-6
Antal sider	6
ISSN	1471-2091
DOI	https://doi.org/10.1186/1471-2091-14-8
Status	Udgivet - 2013

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10.1186/1471-2091-14-8

Nedd8 processing...Forlagets udgivne version, 582 KB

http://dx.doi.org/10.1186/1471-2091-14-8

Citationsformater

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abstract = "Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1. Results: Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo. Conclusions: Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.",

author = "Jean O'Donoghue and Dawadschargal Bech-Otschir and Ida Larsen and Mairi Wallace and Rasmus Hartmann-Petersen and Colin Gordon",

year = "2013",

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T1 - Nedd8 processing enzymes in Schizosaccharomyces pombe

AU - O'Donoghue, Jean

AU - Bech-Otschir, Dawadschargal

AU - Larsen, Ida

AU - Wallace, Mairi

AU - Hartmann-Petersen, Rasmus

AU - Gordon, Colin

PY - 2013

Y1 - 2013

N2 - Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1. Results: Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo. Conclusions: Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.

AB - Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a precursor that must first be processed before it becomes active. In Saccharomyces cerevisiae this is carried out exclusively by the enzyme Yuh1. Results: Here we show that in the fission yeast, Schizosaccharomyces pombe, the Yuh1 orthologue, Uch1, is not the sole Nedd8 processing enzyme. Instead it appears that deubiquitylating enzymes can efficiently process the Nedd8 precursor in vivo. Conclusions: Several enzymes contribute to Nedd8 precursor processing including a number of deubiquitylating enzymes.

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DO - 10.1186/1471-2091-14-8

M3 - Journal article

C2 - 23496905

SN - 1471-2091

VL - 14

SP - 1

EP - 6

JO - BMC Chemical Biology

JF - BMC Chemical Biology

IS - 8

ER -

Nedd8 processing enzymes in Schizosaccharomyces pombe

Abstract

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