Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI

Patrick Rosendahl Andreassen; Sofie Fredberg; Mattias Horan; Maria Højberg Knudsen; Kirstine Jacobsen; Andreas Kjær; Thøger Jensen Krogh; Tina Kronborg; Nicky Cordua Mattsson; Sissel Ida Schmidt; Heidi Wille; Ann Andersen

doi:10.1021/sb500031h

Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI

Patrick Rosendahl Andreassen, Sofie Fredberg, Mattias Horan, Maria Højberg Knudsen, Kirstine Jacobsen, Andreas Kjær, Thøger Jensen Krogh, Tina Kronborg, Nicky Cordua Mattsson, Sissel Ida Schmidt, Heidi Wille, Ann Andersen

Abstract

The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

Originalsprog	Engelsk
Tidsskrift	A C S Synthetic Biology
Vol/bind	3
Udgave nummer	12
Sider (fra-til)	949-52
Antal sider	4
ISSN	2161-5063
DOI	https://doi.org/10.1021/sb500031h
Status	Udgivet - 19 dec. 2014
Udgivet eksternt	Ja

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10.1021/sb500031h

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title = "Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI",

abstract = "The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.",

author = "Andreassen, {Patrick Rosendahl} and Sofie Fredberg and Mattias Horan and Knudsen, {Maria H{\o}jberg} and Kirstine Jacobsen and Andreas Kj{\ae}r and Krogh, {Th{\o}ger Jensen} and Tina Kronborg and Mattsson, {Nicky Cordua} and Schmidt, {Sissel Ida} and Heidi Wille and Ann Andersen",

year = "2014",

month = dec,

day = "19",

doi = "10.1021/sb500031h",

language = "English",

volume = "3",

pages = "949--52",

journal = "ACS Synthetic Biology",

issn = "2161-5063",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI

AU - Andreassen, Patrick Rosendahl

AU - Fredberg, Sofie

AU - Horan, Mattias

AU - Knudsen, Maria Højberg

AU - Jacobsen, Kirstine

AU - Kjær, Andreas

AU - Krogh, Thøger Jensen

AU - Kronborg, Tina

AU - Mattsson, Nicky Cordua

AU - Schmidt, Sissel Ida

AU - Wille, Heidi

AU - Andersen, Ann

PY - 2014/12/19

Y1 - 2014/12/19

N2 - The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

AB - The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

U2 - 10.1021/sb500031h

DO - 10.1021/sb500031h

M3 - Journal article

C2 - 25524095

SN - 2161-5063

VL - 3

SP - 949

EP - 952

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

IS - 12

ER -

Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI

Abstract

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