Naphthylamidase used as a lysosome marker in the study of acute selective necrosis of the internal granular layer of cerebellum.

TY - JOUR

T1 - Naphthylamidase used as a lysosome marker in the study of acute selective necrosis of the internal granular layer of cerebellum.

AU - Albrechtsen, R

N1 - Keywords: Acid Phosphatase; Aged; Amino Acid Naphthylamidases; Autolysis; Cerebellar Cortex; Cytoplasmic Granules; Female; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Lipofuscin; Lysosomes; Male; Middle Aged; Necrosis; Postmortem Changes; Purkinje Cells

PY - 1977

Y1 - 1977

N2 - Histochemical examination of the activity of naphthylamidase (LNAse) in the cerebellar cortex of 70 human autopsies consistantly revealed a marked activity mainly in the internal granular layer with pH optimum of 5.8. Slight enzyme activity was also localized in sites corresponding to lipofuscin deposits and areas of acid phosphatase activity in the Bergmann glial cells, Purkinje cells and in perivascular cells. The histochemical findings support the LNAse reaction as a lysosome marker. Differences in localization of LNAse and acid phosphatase could possibly be due to prior release of the latter enzyme from the internal granular layer. Significant correlation between demonstrable loss of granule cell nuclei (the so-called acute, selective necrosis of the granular layer) and low pH of the cerebellar tissue could be demonstrated in 21 cases. The present findings support the hypothesis that an enzymatic disintegration of the granule cells takes place in postmortem cerebella with low pH simulating a necrotic vital phenomenon.

AB - Histochemical examination of the activity of naphthylamidase (LNAse) in the cerebellar cortex of 70 human autopsies consistantly revealed a marked activity mainly in the internal granular layer with pH optimum of 5.8. Slight enzyme activity was also localized in sites corresponding to lipofuscin deposits and areas of acid phosphatase activity in the Bergmann glial cells, Purkinje cells and in perivascular cells. The histochemical findings support the LNAse reaction as a lysosome marker. Differences in localization of LNAse and acid phosphatase could possibly be due to prior release of the latter enzyme from the internal granular layer. Significant correlation between demonstrable loss of granule cell nuclei (the so-called acute, selective necrosis of the granular layer) and low pH of the cerebellar tissue could be demonstrated in 21 cases. The present findings support the hypothesis that an enzymatic disintegration of the granule cells takes place in postmortem cerebella with low pH simulating a necrotic vital phenomenon.

M3 - Journal article

C2 - 23648

SN - 0365-4184

VL - 85

SP - 875

EP - 888

JO - Acta Pathologica et Microbiologica Scandinavica - Section A Pathology

JF - Acta Pathologica et Microbiologica Scandinavica - Section A Pathology

IS - 6

ER -