Nanowire-aperture probe: local enhanced fluorescence detection for the investigation of live cells at the nanoscale

Rune Schøneberg Frederiksen; Esther Alarcon-llado; Peter Krogstrup; Laura Bojarskaite; Nina Buch-Månson; Jessica Bolinsson; Jesper Nygård; Anna Fontcuberta i Morral; Karen Laurence Martinez

doi:10.1021/acsphotonics.6b00126

Nanowire-aperture probe: local enhanced fluorescence detection for the investigation of live cells at the nanoscale

Rune Schøneberg Frederiksen, Esther Alarcon-llado, Peter Krogstrup, Laura Bojarskaite, Nina Buch-Månson, Jessica Bolinsson, Jesper Nygård, Anna Fontcuberta i Morral, Karen Laurence Martinez

16 Citationer (Scopus)

Abstract

Fluorescence microscopy has tackled many of the burning questions in cellular biology. Probing low-affinity cellular interactions remains one of the major challenges in the field to better understand cellular signaling. We introduce a novel approach - the nanowire-aperture probe (NAP) - to resolve biological signatures with a nanoscale resolution and a boost in light detection. The NAP takes advantage of the photonic properties of semiconductor nanowires and provides a highly localized excitation volume close to the nanowire surface. The probing region extends less than 20 nm into the solution, which can be exploited as a local light probe in fluorescence microscopy. This confined detection volume is especially advantageous in the study of cellular signaling at the cell membrane, as it wraps tightly around the nanowire. The nanowire acts as a local nanoaperture, both focusing the incoming excitation light and guiding photons emitted by the fluorophore. We demonstrate a 20-fold boost in signal-to-background sensitivity for single fluorophores and membrane-localized proteins in live cells. This work opens a completely new avenue for next-generation studies of live cells.

Originalsprog	Engelsk
Tidsskrift	ACS Photonics
Vol/bind	3
Udgave nummer	7
Sider (fra-til)	1208-1216
Antal sider	9
ISSN	2330-4022
DOI	https://doi.org/10.1021/acsphotonics.6b00126
Status	Udgivet - 20 jul. 2016

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10.1021/acsphotonics.6b00126

acsphotonics.6b00126Forlagets udgivne version, 4,73 MB

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abstract = "Fluorescence microscopy has tackled many of the burning questions in cellular biology. Probing low-affinity cellular interactions remains one of the major challenges in the field to better understand cellular signaling. We introduce a novel approach - the nanowire-aperture probe (NAP) - to resolve biological signatures with a nanoscale resolution and a boost in light detection. The NAP takes advantage of the photonic properties of semiconductor nanowires and provides a highly localized excitation volume close to the nanowire surface. The probing region extends less than 20 nm into the solution, which can be exploited as a local light probe in fluorescence microscopy. This confined detection volume is especially advantageous in the study of cellular signaling at the cell membrane, as it wraps tightly around the nanowire. The nanowire acts as a local nanoaperture, both focusing the incoming excitation light and guiding photons emitted by the fluorophore. We demonstrate a 20-fold boost in signal-to-background sensitivity for single fluorophores and membrane-localized proteins in live cells. This work opens a completely new avenue for next-generation studies of live cells.",

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AU - Frederiksen, Rune Schøneberg

AU - Alarcon-llado, Esther

AU - Krogstrup, Peter

AU - Bojarskaite, Laura

AU - Buch-Månson, Nina

AU - Bolinsson, Jessica

AU - Nygård, Jesper

AU - Fontcuberta i Morral, Anna

AU - Martinez, Karen Laurence

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N2 - Fluorescence microscopy has tackled many of the burning questions in cellular biology. Probing low-affinity cellular interactions remains one of the major challenges in the field to better understand cellular signaling. We introduce a novel approach - the nanowire-aperture probe (NAP) - to resolve biological signatures with a nanoscale resolution and a boost in light detection. The NAP takes advantage of the photonic properties of semiconductor nanowires and provides a highly localized excitation volume close to the nanowire surface. The probing region extends less than 20 nm into the solution, which can be exploited as a local light probe in fluorescence microscopy. This confined detection volume is especially advantageous in the study of cellular signaling at the cell membrane, as it wraps tightly around the nanowire. The nanowire acts as a local nanoaperture, both focusing the incoming excitation light and guiding photons emitted by the fluorophore. We demonstrate a 20-fold boost in signal-to-background sensitivity for single fluorophores and membrane-localized proteins in live cells. This work opens a completely new avenue for next-generation studies of live cells.

AB - Fluorescence microscopy has tackled many of the burning questions in cellular biology. Probing low-affinity cellular interactions remains one of the major challenges in the field to better understand cellular signaling. We introduce a novel approach - the nanowire-aperture probe (NAP) - to resolve biological signatures with a nanoscale resolution and a boost in light detection. The NAP takes advantage of the photonic properties of semiconductor nanowires and provides a highly localized excitation volume close to the nanowire surface. The probing region extends less than 20 nm into the solution, which can be exploited as a local light probe in fluorescence microscopy. This confined detection volume is especially advantageous in the study of cellular signaling at the cell membrane, as it wraps tightly around the nanowire. The nanowire acts as a local nanoaperture, both focusing the incoming excitation light and guiding photons emitted by the fluorophore. We demonstrate a 20-fold boost in signal-to-background sensitivity for single fluorophores and membrane-localized proteins in live cells. This work opens a completely new avenue for next-generation studies of live cells.

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DO - 10.1021/acsphotonics.6b00126

M3 - Journal article

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EP - 1216

JO - ACS Photonics

JF - ACS Photonics

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ER -

Nanowire-aperture probe: local enhanced fluorescence detection for the investigation of live cells at the nanoscale

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