Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastoma multiforme cells

TY - JOUR

T1 - Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastoma multiforme cells

AU - Hinzmann, Mateusz

AU - Jaworski, Sławomir

AU - Kutwin, Marta

AU - Jagiełło, Joanna

AU - Koziński, Rafał

AU - Wierzbicki, Mateusz

AU - Grodzik, Marta

AU - Lipińska, Ludwika

AU - Sawosz, Ewa

AU - Chwalibog, André

PY - 2014

Y1 - 2014

N2 - The carbon-based nanomaterial family consists of nanoparticles containing allotropes of carbon, which may have a number of interactions with biological systems. The objective of this study was to evaluate the toxicity of nanoparticles comprised evaluation of cell morphology, and assessment of cell viability by Trypan blue assay and level of DNA fragmentation of U87 cells after 24 hours of incubation with 50 μg/mL carbon nanoparticles. DNA fragmentation was studied using single-cell gel electrophoresis. Incubation with nanoparticles containing the allotropes of carbon did not alter the morphology of the U87 cancer cells. However, incubation with pristine graphene and reduced graphene oxide led to a significant decrease in cell viability, whereas incubation with graphene oxide, graphite, and ultradispersed detonation diamond led to a smaller decrease in cell viability. The results of a comet assay demonstrated that pristine graphene, reduced graphene oxide, graphite, and ultradispersed detonation diamond caused DNA damage and were therefore genotoxic in U87 cells, whereas graphene oxide was not.

AB - The carbon-based nanomaterial family consists of nanoparticles containing allotropes of carbon, which may have a number of interactions with biological systems. The objective of this study was to evaluate the toxicity of nanoparticles comprised evaluation of cell morphology, and assessment of cell viability by Trypan blue assay and level of DNA fragmentation of U87 cells after 24 hours of incubation with 50 μg/mL carbon nanoparticles. DNA fragmentation was studied using single-cell gel electrophoresis. Incubation with nanoparticles containing the allotropes of carbon did not alter the morphology of the U87 cancer cells. However, incubation with pristine graphene and reduced graphene oxide led to a significant decrease in cell viability, whereas incubation with graphene oxide, graphite, and ultradispersed detonation diamond led to a smaller decrease in cell viability. The results of a comet assay demonstrated that pristine graphene, reduced graphene oxide, graphite, and ultradispersed detonation diamond caused DNA damage and were therefore genotoxic in U87 cells, whereas graphene oxide was not.

KW - Diamond

KW - Genotoxicity

KW - Glioblastoma multiforme

KW - Graphene

KW - Graphite

KW - Nanostructures

U2 - 10.2147/IJN.S62497

DO - 10.2147/IJN.S62497

M3 - Journal article

C2 - 24876774

AN - SCOPUS:84900844404

SN - 1176-9114

VL - 9

SP - 2409

EP - 2417

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

IS - 1

ER -