Muscle metabolism during graded quadriceps exercise in man

TY - JOUR

T1 - Muscle metabolism during graded quadriceps exercise in man

AU - Helge, Jørn W

AU - Stallknecht, Bente

AU - Galbo, Henrik

AU - Kiens, Bente

AU - Richter, Erik

N1 - Keywords: Adult; Carbohydrate Metabolism; Carbon Isotopes; Epinephrine; Exercise; Glucose; Glycerol; Glycogenolysis; Humans; Insulin; Lipid Metabolism; Male; Muscle Contraction; Norepinephrine; Oxidation-Reduction; Oxygen Consumption; Palmitic Acid; Physical Endurance; Quadriceps Muscle; Regional Blood Flow

PY - 2007

Y1 - 2007

N2 - The aim of the study was to examine local muscle metabolism in response to graded exercise when the involved muscle mass is too small to elicit marked hormonal changes and local blood flow restriction. Nine healthy overnight fasted male subjects performed knee extension exercise with both thighs kicking at 25% of maximal power (Wmax) for 45 min (23+/-1% of pulmonary) followed by 35 min of kicking with one thigh at 65% and the other at 85% W(max) (40+/-1% ). Primed constant infusion of [U-13C] palmitate and [2H5]glycerol was carried out. Blood was sampled from a femoral artery and both femoral veins, and thigh blood flow was determined by thermodilution. Muscle biopsies were obtained from m. vastus lateralis of both thighs. From rest through exercise at 25, 65 and 85% Wmax the thigh blood flow (0.3+/-0.1, 2.5+/-0.2, 3.5+/-0.2, 4.1+/-0.3 l min(-1)) and oxygen uptake (0.02+/-0.01, 0.27+/-0.03, 0.48+/-0.04, 0.55+/-0.05 l min(-1)) increased (P<0.05). The plasma fatty acids oxidized in the thigh (5+/-1, 114+/-15, 162+/-30, 180+/-31 micromol min(-1)) increased (P<0.05) with exercise intensity, whereas the total thigh fat oxidation (19+/-6, 312+/-64, 356+/-93, 323+/-120 micromol min(-1)) increased (P<0.05) from rest, but remained unchanged through exercise. The thigh glycerol uptake (1+/-1, 16+/-4, 24+/-10, 39+/-8 micromol min(-1)) increased significantly from rest through exercise at 25-65 and 85% Wmax, respectively. Glucose uptake and glycogen breakdown always increased with exercise intensity. In conclusion, in the presence of a high blood flow and oxygen supply and only small hormonal changes, total fat oxidation in muscle increases from rest to light exercise, but then remains constant with exercise intensity up to heavy exercise. However, with increasing exercise intensity, oxidation of plasma free fatty acids increases and accordingly oxidation of other fat sources decreases. These findings are in contrast to whole body measurements performed during graded exercise involving a large muscle mass during which fat oxidation peaks at around 60% of .

AB - The aim of the study was to examine local muscle metabolism in response to graded exercise when the involved muscle mass is too small to elicit marked hormonal changes and local blood flow restriction. Nine healthy overnight fasted male subjects performed knee extension exercise with both thighs kicking at 25% of maximal power (Wmax) for 45 min (23+/-1% of pulmonary) followed by 35 min of kicking with one thigh at 65% and the other at 85% W(max) (40+/-1% ). Primed constant infusion of [U-13C] palmitate and [2H5]glycerol was carried out. Blood was sampled from a femoral artery and both femoral veins, and thigh blood flow was determined by thermodilution. Muscle biopsies were obtained from m. vastus lateralis of both thighs. From rest through exercise at 25, 65 and 85% Wmax the thigh blood flow (0.3+/-0.1, 2.5+/-0.2, 3.5+/-0.2, 4.1+/-0.3 l min(-1)) and oxygen uptake (0.02+/-0.01, 0.27+/-0.03, 0.48+/-0.04, 0.55+/-0.05 l min(-1)) increased (P<0.05). The plasma fatty acids oxidized in the thigh (5+/-1, 114+/-15, 162+/-30, 180+/-31 micromol min(-1)) increased (P<0.05) with exercise intensity, whereas the total thigh fat oxidation (19+/-6, 312+/-64, 356+/-93, 323+/-120 micromol min(-1)) increased (P<0.05) from rest, but remained unchanged through exercise. The thigh glycerol uptake (1+/-1, 16+/-4, 24+/-10, 39+/-8 micromol min(-1)) increased significantly from rest through exercise at 25-65 and 85% Wmax, respectively. Glucose uptake and glycogen breakdown always increased with exercise intensity. In conclusion, in the presence of a high blood flow and oxygen supply and only small hormonal changes, total fat oxidation in muscle increases from rest to light exercise, but then remains constant with exercise intensity up to heavy exercise. However, with increasing exercise intensity, oxidation of plasma free fatty acids increases and accordingly oxidation of other fat sources decreases. These findings are in contrast to whole body measurements performed during graded exercise involving a large muscle mass during which fat oxidation peaks at around 60% of .

U2 - 10.1113/jphysiol.2007.128348

DO - 10.1113/jphysiol.2007.128348

M3 - Journal article

C2 - 17379639

SN - 0022-3751

VL - 581

SP - 1247

EP - 1258

JO - The Journal of Physiology

JF - The Journal of Physiology

IS - 3

ER -