Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients

TY - JOUR

T1 - Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients

AU - Lee, Baoleri

AU - Schjerling, Charlotte K

AU - Kirkby, Nikolai

AU - Hoffmann, Nadine

AU - Borup, Rehannah

AU - Molin, Søren

AU - Høiby, Niels

AU - Ciofu, Oana

N1 - © 2011 The Authors. APMIS © 2011 APMIS.

PY - 2011/4

Y1 - 2011/4

N2 - Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.

AB - Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.

KW - Alginates

KW - Bacterial Proteins

KW - Biofilms

KW - Chronic Disease

KW - Cystic Fibrosis

KW - Denmark

KW - Gene Expression Profiling

KW - Genes, Bacterial

KW - Genotype

KW - Glucuronic Acid

KW - Hexuronic Acids

KW - Humans

KW - Lung Diseases

KW - Mutation

KW - Oligonucleotide Array Sequence Analysis

KW - Phenotype

KW - Pseudomonas Infections

KW - Pseudomonas aeruginosa

KW - Quorum Sensing

KW - Sigma Factor

KW - Trans-Activators

U2 - 10.1111/j.1600-0463.2011.02726.x

DO - 10.1111/j.1600-0463.2011.02726.x

M3 - Journal article

C2 - 21492226

SN - 0903-465X

VL - 119

SP - 263

EP - 274

JO - APMIS. Supplementum

JF - APMIS. Supplementum

IS - 4-5

ER -