mRNA distribution of CGRP and its receptor components in the trigeminovascular system and other pain related structures in rat brain, and effect of intracerebroventricular administration of CGRP on Fos expression in the TNC

Deepak Kumar Bhatt; Saurabh Gupta; Kenneth B Ploug; Inger Jansen-Olesen; Jes Olesen

doi:10.1016/j.neulet.2013.11.057

mRNA distribution of CGRP and its receptor components in the trigeminovascular system and other pain related structures in rat brain, and effect of intracerebroventricular administration of CGRP on Fos expression in the TNC

Deepak Kumar Bhatt, Saurabh Gupta, Kenneth B Ploug, Inger Jansen-Olesen, Jes Olesen

10 Citationer (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) infusion in humans provokes headache resembling spontaneous migraine, and CGRP receptor antagonists are effective against acute migraine. We hypothesized that CGRP infusion in the lateral ventricle (LV) will induce neuronal activation reflected by increase in Fos expression in the trigeminal nucleus caudalis (TNC). CGRP was infused intracerebroventricularly (i.c.v.) in freely moving rats to circumvent factors like anaesthesia, acute surgery and severe hypotension, three confounding factors for Fos expression. TNCs were isolated 2h after CGRP infusion. The level of Fos protein expression in TNC was analysed by immunohistochemistry (IHC). mRNA expression of CGRP and its receptor components in trigeminovascular and other pain processing structures in the brain was also studied. CGRP i.c.v. infusion did not induce Fos activation in the TNC. mRNA expression profile showed that CGRP and its receptor components were widely distributed in trigeminovascular and other pain processing structures. The widespread presence of CGRP receptor mRNA in the various central pain pathways suggests that CGRP might play a role in migraine pathogenesis.

Originalsprog	Engelsk
Tidsskrift	Neuroscience Letters
Vol/bind	559
Sider (fra-til)	99-104
Antal sider	6
ISSN	0304-3940
DOI	https://doi.org/10.1016/j.neulet.2013.11.057
Status	Udgivet - 24 jan. 2014

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10.1016/j.neulet.2013.11.057

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Citationsformater

Bhatt, D. K., Gupta, S., Ploug, K. B., Jansen-Olesen, I., & Olesen, J. (2014). mRNA distribution of CGRP and its receptor components in the trigeminovascular system and other pain related structures in rat brain, and effect of intracerebroventricular administration of CGRP on Fos expression in the TNC. Neuroscience Letters, 559, 99-104. https://doi.org/10.1016/j.neulet.2013.11.057

mRNA distribution of CGRP and its receptor components in the trigeminovascular system and other pain related structures in rat brain, and effect of intracerebroventricular administration of CGRP on Fos expression in the TNC. / Bhatt, Deepak Kumar; Gupta, Saurabh; Ploug, Kenneth B et al.
I: Neuroscience Letters, Bind 559, 24.01.2014, s. 99-104.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Bhatt, DK, Gupta, S, Ploug, KB, Jansen-Olesen, I & Olesen, J 2014, 'mRNA distribution of CGRP and its receptor components in the trigeminovascular system and other pain related structures in rat brain, and effect of intracerebroventricular administration of CGRP on Fos expression in the TNC', Neuroscience Letters, bind 559, s. 99-104. https://doi.org/10.1016/j.neulet.2013.11.057

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title = "mRNA distribution of CGRP and its receptor components in the trigeminovascular system and other pain related structures in rat brain, and effect of intracerebroventricular administration of CGRP on Fos expression in the TNC",

abstract = "Calcitonin gene-related peptide (CGRP) infusion in humans provokes headache resembling spontaneous migraine, and CGRP receptor antagonists are effective against acute migraine. We hypothesized that CGRP infusion in the lateral ventricle (LV) will induce neuronal activation reflected by increase in Fos expression in the trigeminal nucleus caudalis (TNC). CGRP was infused intracerebroventricularly (i.c.v.) in freely moving rats to circumvent factors like anaesthesia, acute surgery and severe hypotension, three confounding factors for Fos expression. TNCs were isolated 2h after CGRP infusion. The level of Fos protein expression in TNC was analysed by immunohistochemistry (IHC). mRNA expression of CGRP and its receptor components in trigeminovascular and other pain processing structures in the brain was also studied. CGRP i.c.v. infusion did not induce Fos activation in the TNC. mRNA expression profile showed that CGRP and its receptor components were widely distributed in trigeminovascular and other pain processing structures. The widespread presence of CGRP receptor mRNA in the various central pain pathways suggests that CGRP might play a role in migraine pathogenesis.",

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AU - Bhatt, Deepak Kumar

AU - Gupta, Saurabh

AU - Ploug, Kenneth B

AU - Jansen-Olesen, Inger

AU - Olesen, Jes

PY - 2014/1/24

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N2 - Calcitonin gene-related peptide (CGRP) infusion in humans provokes headache resembling spontaneous migraine, and CGRP receptor antagonists are effective against acute migraine. We hypothesized that CGRP infusion in the lateral ventricle (LV) will induce neuronal activation reflected by increase in Fos expression in the trigeminal nucleus caudalis (TNC). CGRP was infused intracerebroventricularly (i.c.v.) in freely moving rats to circumvent factors like anaesthesia, acute surgery and severe hypotension, three confounding factors for Fos expression. TNCs were isolated 2h after CGRP infusion. The level of Fos protein expression in TNC was analysed by immunohistochemistry (IHC). mRNA expression of CGRP and its receptor components in trigeminovascular and other pain processing structures in the brain was also studied. CGRP i.c.v. infusion did not induce Fos activation in the TNC. mRNA expression profile showed that CGRP and its receptor components were widely distributed in trigeminovascular and other pain processing structures. The widespread presence of CGRP receptor mRNA in the various central pain pathways suggests that CGRP might play a role in migraine pathogenesis.

AB - Calcitonin gene-related peptide (CGRP) infusion in humans provokes headache resembling spontaneous migraine, and CGRP receptor antagonists are effective against acute migraine. We hypothesized that CGRP infusion in the lateral ventricle (LV) will induce neuronal activation reflected by increase in Fos expression in the trigeminal nucleus caudalis (TNC). CGRP was infused intracerebroventricularly (i.c.v.) in freely moving rats to circumvent factors like anaesthesia, acute surgery and severe hypotension, three confounding factors for Fos expression. TNCs were isolated 2h after CGRP infusion. The level of Fos protein expression in TNC was analysed by immunohistochemistry (IHC). mRNA expression of CGRP and its receptor components in trigeminovascular and other pain processing structures in the brain was also studied. CGRP i.c.v. infusion did not induce Fos activation in the TNC. mRNA expression profile showed that CGRP and its receptor components were widely distributed in trigeminovascular and other pain processing structures. The widespread presence of CGRP receptor mRNA in the various central pain pathways suggests that CGRP might play a role in migraine pathogenesis.

KW - Animals

KW - Calcitonin Gene-Related Peptide

KW - Gene Expression Regulation

KW - Genes, fos

KW - Infusions, Intraventricular

KW - Male

KW - Pain

KW - RNA, Messenger

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptors, Calcitonin Gene-Related Peptide

KW - Tissue Distribution

KW - Trigeminal Nuclei

U2 - 10.1016/j.neulet.2013.11.057

DO - 10.1016/j.neulet.2013.11.057

M3 - Journal article

C2 - 24321404

SN - 0304-3940

VL - 559

SP - 99

EP - 104

JO - Neuroscience Letters

JF - Neuroscience Letters

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