Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status

TY - JOUR

T1 - Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status

AU - Persson, Daniel Olof

AU - de Bang, Thomas Christian

AU - Pedas, Pai Rosager

AU - Kutman, Umit Baris

AU - Cakmak, Ismail

AU - Andersen, Birgit

AU - Finnie, Christine

AU - Schjørring, Jan Kofod

AU - Husted, Søren

N1 - © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Low concentration of zinc (Zn) in the endosperm of cereals is a major factor contributing to Zn deficiency in human populations. We have investigated how combined Zn and nitrogen (N) fertilization affects the speciation and localization of Zn in durum wheat (Triticum durum). Zn-binding proteins were analysed with liquid chromatography ICP-MS and Orbitrap MS(2) , respectively. Laser ablation ICP-MS with simultaneous Zn, sulphur (S) and phosphorus (P) detection was used for bioimaging of Zn and its potential ligands. Increasing the Zn and N supply had a major impact on the Zn concentration in the endosperm, reaching concentrations higher than current breeding targets. The S concentration also increased, but S was only partly co-localized with Zn. The mutual Zn and S enrichment was reflected in substantially more Zn bound to small cysteine-rich proteins (apparent size 10-30 kDa), whereas the response of larger proteins (apparent size > 50 kDa) was only modest. Most of the Zn-responsive proteins were associated with redox- and stress-related processes. This study offers a methodological platform to deepen the understanding of processes behind endosperm Zn enrichment. Novel information is provided on how the localization and speciation of Zn is modified during Zn biofortification of grains.

AB - Low concentration of zinc (Zn) in the endosperm of cereals is a major factor contributing to Zn deficiency in human populations. We have investigated how combined Zn and nitrogen (N) fertilization affects the speciation and localization of Zn in durum wheat (Triticum durum). Zn-binding proteins were analysed with liquid chromatography ICP-MS and Orbitrap MS(2) , respectively. Laser ablation ICP-MS with simultaneous Zn, sulphur (S) and phosphorus (P) detection was used for bioimaging of Zn and its potential ligands. Increasing the Zn and N supply had a major impact on the Zn concentration in the endosperm, reaching concentrations higher than current breeding targets. The S concentration also increased, but S was only partly co-localized with Zn. The mutual Zn and S enrichment was reflected in substantially more Zn bound to small cysteine-rich proteins (apparent size 10-30 kDa), whereas the response of larger proteins (apparent size > 50 kDa) was only modest. Most of the Zn-responsive proteins were associated with redox- and stress-related processes. This study offers a methodological platform to deepen the understanding of processes behind endosperm Zn enrichment. Novel information is provided on how the localization and speciation of Zn is modified during Zn biofortification of grains.

KW - Journal Article

U2 - 10.1111/nph.13989

DO - 10.1111/nph.13989

M3 - Journal article

C2 - 27159614

SN - 1469-8137

VL - 211

SP - 1255

EP - 1265

JO - New Phytologist

JF - New Phytologist

IS - 4

ER -