TY - JOUR
T1 - Molecular identification of the first insect ecdysis triggering hormone receptors.
AU - Iversen, Annette
AU - Cazzamali, Giuseppe
AU - Williamson, Michael
AU - Hauser, Frank
AU - Grimmelikhuijzen, Cornelis J P
N1 - Keywords: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; CHO Cells; Cricetinae; Drosophila; Drosophila Proteins; Insect Hormones; Molecular Sequence Data; Molting; RNA, Messenger; Receptors, Cell Surface; Receptors, G-Protein-Coupled; Receptors, Neuropeptide; Sequence Homology, Amino Acid
PY - 2002
Y1 - 2002
N2 - The Drosophila Genome Project website (www.flybase.org) contains an annotated gene sequence (CG5911), coding for a G protein-coupled receptor. We cloned the cDNA corresponding to this sequence and found that the gene has not been correctly predicted. The corrected gene CG5911 has five introns and six exons (1-6). Alternative splicing yields two cDNAs called A (containing exons 1-5) and B (containing exons 1-4, 6). We expressed these splicing variants in Chinese hamster ovary cells and found that the corrected CG5911-A and -B cDNAs coded for two different G protein-coupled receptors that could be activated by low concentrations of Drosophila ecdysis triggering hormones-1 and -2. Ecdysis (cuticle shedding) is an important behaviour, allowing growth and metamorphosis in insects and other arthropods. Our paper is the first report on the molecular identification of ecdysis triggering hormone receptors from insects.
AB - The Drosophila Genome Project website (www.flybase.org) contains an annotated gene sequence (CG5911), coding for a G protein-coupled receptor. We cloned the cDNA corresponding to this sequence and found that the gene has not been correctly predicted. The corrected gene CG5911 has five introns and six exons (1-6). Alternative splicing yields two cDNAs called A (containing exons 1-5) and B (containing exons 1-4, 6). We expressed these splicing variants in Chinese hamster ovary cells and found that the corrected CG5911-A and -B cDNAs coded for two different G protein-coupled receptors that could be activated by low concentrations of Drosophila ecdysis triggering hormones-1 and -2. Ecdysis (cuticle shedding) is an important behaviour, allowing growth and metamorphosis in insects and other arthropods. Our paper is the first report on the molecular identification of ecdysis triggering hormone receptors from insects.
U2 - 10.1016/S0006-291X(02)02798-5
DO - 10.1016/S0006-291X(02)02798-5
M3 - Journal article
C2 - 12470668
SN - 0006-291X
VL - 299
SP - 924
EP - 931
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 5
ER -