TY - JOUR
T1 - Molecular evolution of GB virus B hepatitis virus during acute resolving and persistent infections in experimentally infected tamarins
AU - Takikawa, Shingo
AU - Engle, Ronald E
AU - Faulk, Kristina N
AU - Emerson, Suzanne U
AU - Purcell, Robert H
AU - Bukh, Jens
N1 - Keywords: Amino Acid Substitution; Animals; Disease Models, Animal; Evolution, Molecular; GB virus B; Gene Products, pol; Hepatitis, Viral, Animal; Leontopithecus; Monkey Diseases
PY - 2010
Y1 - 2010
N2 - GB virus B (GBV-B) causes acute hepatitis in experimentally infected tamarins. We compared evolutionary features in acute resolving and persistent GBV-B infection. We detected no evidence of evolution in four animals with clearance during weeks 9-12, whereas three animals with clearance during weeks 13-26 had several substitutions in their polyprotein sequence. A single tamarin had long-term GBV-B viraemia; analysis of virus recovered at weeks 2, 5, 12, 20, 26, 52 and 104 demonstrated that mutations accumulated over time. Overall, the amino acid substitution rate was 3.5×10-3 and 1.1×10-3 substitutions per site year-1 during weeks 1-52 and 53-104, respectively. Thus, there was a significant decrease in evolution over time, as found for hepatitis C virus. The rate of non-synonymous substitution per non-synonymous site compared with that of synonymous substitution per synonymous site decreased over time, suggesting reduction of positive selective pressure. These data demonstrate that prolonged GBV-B infection is associated with viral evolution.
AB - GB virus B (GBV-B) causes acute hepatitis in experimentally infected tamarins. We compared evolutionary features in acute resolving and persistent GBV-B infection. We detected no evidence of evolution in four animals with clearance during weeks 9-12, whereas three animals with clearance during weeks 13-26 had several substitutions in their polyprotein sequence. A single tamarin had long-term GBV-B viraemia; analysis of virus recovered at weeks 2, 5, 12, 20, 26, 52 and 104 demonstrated that mutations accumulated over time. Overall, the amino acid substitution rate was 3.5×10-3 and 1.1×10-3 substitutions per site year-1 during weeks 1-52 and 53-104, respectively. Thus, there was a significant decrease in evolution over time, as found for hepatitis C virus. The rate of non-synonymous substitution per non-synonymous site compared with that of synonymous substitution per synonymous site decreased over time, suggesting reduction of positive selective pressure. These data demonstrate that prolonged GBV-B infection is associated with viral evolution.
U2 - 10.1099/vir.0.015750-0
DO - 10.1099/vir.0.015750-0
M3 - Journal article
C2 - 19906942
SN - 0022-1317
VL - 91
SP - 727
EP - 733
JO - Journal of General Virology
JF - Journal of General Virology
IS - Pt 3
ER -
