Molecular Cloning, Genomic Organization and Developmental Regulation of a Novel Receptor from Drosophila melanogaster Structurally Related to Gonadotropin-Releasing Hormone Receptors from Vertebrates

TY - JOUR

T1 - Molecular Cloning, Genomic Organization and Developmental Regulation of a Novel Receptor from Drosophila melanogaster Structurally Related to Gonadotropin-Releasing Hormone Receptors from Vertebrates

AU - Hauser, Frank

AU - Søndergaard, Leif

AU - Grimmelikhuijzen, Cornelis J.P.

PY - 1998/8/28

Y1 - 1998/8/28

N2 - After screening the data base of the BerkeleyDrosophilaGenome Project with a sequence coding for the transmembrane region of a G protein-coupled receptor, we found thatDrosophilamight contain a gene coding for a receptor that is structurally related to the Gonadotropin-Releasing Hormone (GnRH) receptors from vertebrates. Using the polymerase chain reaction, withDrosophilacDNA as a template, and oligonucleotide probes coding for the presumed exons of this gene, we were able to clone the cDNA coding for this receptor. The transmembrane region of the receptor shows 36% amino acid residue identity with the transmembrane region of the catfish and 31% amino acid residue identity with that of the rat GnRH receptor. TheDrosophilareceptor gene contains six introns, whereas the rat gene contains two; one intron in theDrosophilagene occurs at exactly the same position and has the same intron phasing as one intron in the rat gene, suggesting that theDrosophilaand mammalian GnRH receptor genes are evolutionarily related. Northern blot analyses show that theDrosophilareceptor gene is progressively expressed during larval development with a prominent maximum at the 3rd instar larval stage. Pupae contain low amounts of receptor mRNA, while adult flies contain higher levels, with males having about five times more receptor mRNA than female flies. Southern blot analyses show thatDrosophilacontains only one copy of the receptor gene, which is located at position 27A2-B1 of chromosome 2. This paper is the first report on the molecular cloning of a member of the GnRH receptor family from invertebrates.

AB - After screening the data base of the BerkeleyDrosophilaGenome Project with a sequence coding for the transmembrane region of a G protein-coupled receptor, we found thatDrosophilamight contain a gene coding for a receptor that is structurally related to the Gonadotropin-Releasing Hormone (GnRH) receptors from vertebrates. Using the polymerase chain reaction, withDrosophilacDNA as a template, and oligonucleotide probes coding for the presumed exons of this gene, we were able to clone the cDNA coding for this receptor. The transmembrane region of the receptor shows 36% amino acid residue identity with the transmembrane region of the catfish and 31% amino acid residue identity with that of the rat GnRH receptor. TheDrosophilareceptor gene contains six introns, whereas the rat gene contains two; one intron in theDrosophilagene occurs at exactly the same position and has the same intron phasing as one intron in the rat gene, suggesting that theDrosophilaand mammalian GnRH receptor genes are evolutionarily related. Northern blot analyses show that theDrosophilareceptor gene is progressively expressed during larval development with a prominent maximum at the 3rd instar larval stage. Pupae contain low amounts of receptor mRNA, while adult flies contain higher levels, with males having about five times more receptor mRNA than female flies. Southern blot analyses show thatDrosophilacontains only one copy of the receptor gene, which is located at position 27A2-B1 of chromosome 2. This paper is the first report on the molecular cloning of a member of the GnRH receptor family from invertebrates.

U2 - 10.1006/bbrc.1998.9230

DO - 10.1006/bbrc.1998.9230

M3 - Journal article

SN - 0006-291X

VL - 249

SP - 822

EP - 828

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -