@article{48b127c0526f11dd8d9f000ea68e967b,
title = "Molecular characterization of a novel human hybrid-type receptor that binds the alpha2-macroglobulin receptor-associated protein.",
abstract = "The 39-40-kDa receptor-associated protein (RAP) binds to the members of the low density lipoprotein receptor gene family and functions as a specialized endoplasmic reticulum/Golgi chaperone. Using RAP affinity chromatography, we have purified a novel approximately 250-kDa brain protein and isolated the corresponding cDNA. The gene, designated SORL1, maps to chromosome 11q 23/24 and encodes a 2214-residue type 1 receptor containing a furin cleavage site immediately preceding the N terminus determined in the purified protein. The receptor, designated sorLA-1, has a short cytoplasmic tail containing a tyrosine-based internalization signal and a large external part containing (from the N-terminal): 1) a segment homologous to domains in the yeast vacuolar protein sorting 10 protein, Vps10p, that binds carboxypeptidase Y, 2) five tandemly arranged YWTD repeats and a cluster of 11 class A repeats characteristic of the low density lipoprotein receptor gene family receptors, and 3) six tandemly arranged fibronectin type III repeats also found in certain neural adhesion proteins. sorLA-1 may therefore be classified as a hybrid receptor. Northern blotting revealed specific mRNA transcripts in brain, spinal cord, and testis but not in several major organs. Both RAP and an antibody against a synthetic peptide derived from a sequence determined in the mature protein detected sorLA-1 in crude human brain extracts. The domain structure suggests that sorLA-1 is an endocytic receptor possibly implicated in the uptake of lipoproteins and of proteases.",
author = "Linda Jacobsen and P Madsen and Moestrup, {S K} and Lund, {Anders Henrik} and Niels Tommerup and A Nykjaer and L Sottrup-Jensen and J Gliemann and Petersen, {C M}",
note = "Keywords: Amino Acid Sequence; Animals; Blotting, Northern; Caenorhabditis elegans; Carrier Proteins; Chromosome Mapping; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Heymann Nephritis Antigenic Complex; Humans; LDL-Receptor Related Protein-Associated Protein; LDL-Receptor Related Proteins; Membrane Glycoproteins; Membrane Transport Proteins; Molecular Chaperones; Molecular Sequence Data; Rats; Receptors, Cell Surface; Receptors, LDL; Tissue Distribution",
year = "1996",
language = "English",
volume = "271",
pages = "31379--83",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "49",
}