Molecular basis for the presence of glycosylated onco-foetal fibronectin in oral carcinomas: the production of glycosylated onco-foetal fibronectin by carcinoma cells

TY - JOUR

T1 - Molecular basis for the presence of glycosylated onco-foetal fibronectin in oral carcinomas

T2 - the production of glycosylated onco-foetal fibronectin by carcinoma cells

AU - Wandall, Hans H

AU - Dabelsteen, Sally

AU - Sørensen, Jens Ahm

AU - Krogdahl, Annelise

AU - Mandel, Ulla

AU - Dabelsteen, Erik

PY - 2007/3

Y1 - 2007/3

N2 - Glycosylated onco-foetal fibronectin (GOF) deposited in the stroma of oral squamous cell carcinomas correlates with survival. One of the two polypeptide GalNAc-transferases, GalNAc-T3 or GalNAc-T6, is required for the biosynthesis of GOF by the initiation of a unique O-glycan in the alternative spliced IIICS region. Using cell culture experiments, immunohistochemical staining of primary tissue, and RT-PCR of tumour cells isolated by laser capture techniques we have examined the molecular basis for the production of GOF in oral carcinomas. Immuno-histochemical investigation confirmed the stromal deposition of GOF in oral carcinomas. However, neither GalNAc-T3 nor GalNAc-T6 could be detected in stromal fibroblasts. In contrast both transferases were present in the oral squamous carcinoma cells, suggesting that GOF is produced by the oral cancer cells and not only the stromal cells. RT-PCR analysis of RNA isolated from carcinoma cells provided further support for this conclusion by demonstrating in-splicing of the alternative spliced IIICS domain in GOF.

AB - Glycosylated onco-foetal fibronectin (GOF) deposited in the stroma of oral squamous cell carcinomas correlates with survival. One of the two polypeptide GalNAc-transferases, GalNAc-T3 or GalNAc-T6, is required for the biosynthesis of GOF by the initiation of a unique O-glycan in the alternative spliced IIICS region. Using cell culture experiments, immunohistochemical staining of primary tissue, and RT-PCR of tumour cells isolated by laser capture techniques we have examined the molecular basis for the production of GOF in oral carcinomas. Immuno-histochemical investigation confirmed the stromal deposition of GOF in oral carcinomas. However, neither GalNAc-T3 nor GalNAc-T6 could be detected in stromal fibroblasts. In contrast both transferases were present in the oral squamous carcinoma cells, suggesting that GOF is produced by the oral cancer cells and not only the stromal cells. RT-PCR analysis of RNA isolated from carcinoma cells provided further support for this conclusion by demonstrating in-splicing of the alternative spliced IIICS domain in GOF.

KW - Actins

KW - Biocompatible Materials

KW - Carcinoma, Squamous Cell

KW - Cell Line, Tumor

KW - Cells, Cultured

KW - Collagen

KW - Drug Combinations

KW - Fibroblasts

KW - Fibronectins

KW - Glycosylation

KW - Humans

KW - Immunohistochemistry

KW - Keratinocytes

KW - Laminin

KW - Mouth Neoplasms

KW - Muscle, Smooth

KW - Proteoglycans

KW - Sialyltransferases

U2 - 10.1016/j.oraloncology.2006.03.005

DO - 10.1016/j.oraloncology.2006.03.005

M3 - Journal article

C2 - 16857413

SN - 1368-8375

VL - 43

SP - 301

EP - 309

JO - Oral Oncology

JF - Oral Oncology

IS - 3

ER -