microRNA regulation of the embryonic hypoxic response in Caenorhabditis elegans

Konstantinos Kagias; Roger Pocock

doi:10.1038/srep11284

microRNA regulation of the embryonic hypoxic response in Caenorhabditis elegans

Konstantinos Kagias, Roger Pocock

10 Citationer (Scopus)

Abstract

Layered strategies to combat hypoxia provide flexibility in dynamic oxygen environments. Here we show that multiple miRNAs are required for hypoxic survival responses during C. elegans embryogenesis. Certain miRNAs promote while others antagonize the hypoxic survival response. We found that expression of the mir-35 family is regulated by hypoxia in a HIF-1-independent manner and loss of mir-35-41 weakens hypoxic survival mechanisms in embryos. In addition, correct regulation of the RNA binding protein, SUP-26, a mir-35 family target, is needed for survival in chronic hypoxia. The identification of the full mRNA target repertoire of these miRNAs will reveal the miRNA-regulated network of hypoxic survival mechanisms in C. elegans.

Originalsprog	Engelsk
Tidsskrift	Scientific Reports
Vol/bind	5
Sider (fra-til)	11284
Antal sider	9
ISSN	2045-2322
DOI	https://doi.org/10.1038/srep11284
Status	Udgivet - 11 jun. 2015

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10.1038/srep11284

Citationsformater

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title = "microRNA regulation of the embryonic hypoxic response in Caenorhabditis elegans",

abstract = "Layered strategies to combat hypoxia provide flexibility in dynamic oxygen environments. Here we show that multiple miRNAs are required for hypoxic survival responses during C. elegans embryogenesis. Certain miRNAs promote while others antagonize the hypoxic survival response. We found that expression of the mir-35 family is regulated by hypoxia in a HIF-1-independent manner and loss of mir-35-41 weakens hypoxic survival mechanisms in embryos. In addition, correct regulation of the RNA binding protein, SUP-26, a mir-35 family target, is needed for survival in chronic hypoxia. The identification of the full mRNA target repertoire of these miRNAs will reveal the miRNA-regulated network of hypoxic survival mechanisms in C. elegans.",

keywords = "Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Hypoxia, Embryonic Development, MicroRNAs, Oxygen, Promoter Regions, Genetic, RNA-Binding Proteins, Transcription Factors, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",

author = "Konstantinos Kagias and Roger Pocock",

year = "2015",

month = jun,

day = "11",

doi = "10.1038/srep11284",

language = "English",

volume = "5",

pages = "11284",

journal = "Scientific Reports",

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TY - JOUR

T1 - microRNA regulation of the embryonic hypoxic response in Caenorhabditis elegans

AU - Kagias, Konstantinos

AU - Pocock, Roger

PY - 2015/6/11

Y1 - 2015/6/11

N2 - Layered strategies to combat hypoxia provide flexibility in dynamic oxygen environments. Here we show that multiple miRNAs are required for hypoxic survival responses during C. elegans embryogenesis. Certain miRNAs promote while others antagonize the hypoxic survival response. We found that expression of the mir-35 family is regulated by hypoxia in a HIF-1-independent manner and loss of mir-35-41 weakens hypoxic survival mechanisms in embryos. In addition, correct regulation of the RNA binding protein, SUP-26, a mir-35 family target, is needed for survival in chronic hypoxia. The identification of the full mRNA target repertoire of these miRNAs will reveal the miRNA-regulated network of hypoxic survival mechanisms in C. elegans.

AB - Layered strategies to combat hypoxia provide flexibility in dynamic oxygen environments. Here we show that multiple miRNAs are required for hypoxic survival responses during C. elegans embryogenesis. Certain miRNAs promote while others antagonize the hypoxic survival response. We found that expression of the mir-35 family is regulated by hypoxia in a HIF-1-independent manner and loss of mir-35-41 weakens hypoxic survival mechanisms in embryos. In addition, correct regulation of the RNA binding protein, SUP-26, a mir-35 family target, is needed for survival in chronic hypoxia. The identification of the full mRNA target repertoire of these miRNAs will reveal the miRNA-regulated network of hypoxic survival mechanisms in C. elegans.

KW - Animals

KW - Caenorhabditis elegans

KW - Caenorhabditis elegans Proteins

KW - Cell Hypoxia

KW - Embryonic Development

KW - MicroRNAs

KW - Oxygen

KW - Promoter Regions, Genetic

KW - RNA-Binding Proteins

KW - Transcription Factors

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/srep11284

DO - 10.1038/srep11284

M3 - Journal article

C2 - 26063315

SN - 2045-2322

VL - 5

SP - 11284

JO - Scientific Reports

JF - Scientific Reports

ER -

microRNA regulation of the embryonic hypoxic response in Caenorhabditis elegans

Abstract

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