MicroRNA miR-125b induces senescence in human melanoma cells

TY - JOUR

T1 - MicroRNA miR-125b induces senescence in human melanoma cells

AU - Glud, Martin

AU - Manfé, Valentina

AU - Biskup, Edyta

AU - Holst, Line

AU - Dirksen, Anne Marie Ahlburg

AU - Hastrup, Nina

AU - Nielsen, Finn C

AU - Drzewiecki, Krzysztof T

AU - Gniadecki, Robert

PY - 2011/6/1

Y1 - 2011/6/1

N2 - MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic β-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor.

AB - MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic β-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor.

U2 - 10.1097/cmr.0b013e328345333b

DO - 10.1097/cmr.0b013e328345333b

M3 - Journal article

C2 - 21460750

SN - 0960-8931

VL - 21

SP - 253

EP - 256

JO - Melanoma Research

JF - Melanoma Research

IS - 3

ER -