MicroRNA expression analysis by LNA enhanced microarrays

TY - CHAP

T1 - MicroRNA expression analysis by LNA enhanced microarrays

AU - Søkilde, Rolf

AU - Kaczkowski, Bogumil

AU - Barken, Kim Bundvig

AU - Mouritzen, Peter

AU - Møller, Søren

AU - Litman, Thomas

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Array expression profiling of miRNAs has become a widely used technique and thereby gained a broader user spectrum from novice to highly experienced array specialists. In this chapter we discuss the challenges in designing microarrays for miRNA detection and show the advantages of LNA™-enhanced capture probes. We cover important aspects of array normalization and show its practical application in a case study with miRNA data from the NCI-60 cell line panel. We also demonstrate that commonly used methods for normalization of mRNA expression arrays can be applied for miRNA array data. These methods are implemented in both open source and commercial software.

AB - Array expression profiling of miRNAs has become a widely used technique and thereby gained a broader user spectrum from novice to highly experienced array specialists. In this chapter we discuss the challenges in designing microarrays for miRNA detection and show the advantages of LNA™-enhanced capture probes. We cover important aspects of array normalization and show its practical application in a case study with miRNA data from the NCI-60 cell line panel. We also demonstrate that commonly used methods for normalization of mRNA expression arrays can be applied for miRNA array data. These methods are implemented in both open source and commercial software.

UR - http://www.scopus.com/inward/record.url?scp=79955747768&partnerID=8YFLogxK

U2 - 10.4032/9789814267540

DO - 10.4032/9789814267540

M3 - Book chapter

AN - SCOPUS:79955747768

SN - 9789814267014

SP - 23

EP - 46

BT - MicroRNA Profiling in Cancer

PB - Pan Stanford Publishing Pte. Ltd.

ER -