Abstract
Background: Psoriasis is a systemic inflammatory skin disease. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that recently have been found in the blood to be relevant as disease biomarkers. Objective: We aimed to explore miRNAs potential as blood biomarkers for psoriasis. Methods: Using microarray and quantitative real-time PCR we measured the global miRNA expression in whole blood, plasma and peripheral blood mononuclear cells (PBMCs) from patients with psoriasis and healthy controls. Results: We identified several deregulated miRNAs in the blood from patients with psoriasis including miR-223 and miR-143 which were found to be significantly upregulated in the PBMCs from patients with psoriasis compared with healthy controls (FCH. =. 1.63, P<. 0.01; FCH. =. 2.18, P<. 0.01, respectively). In addition, miR-223 and miR-143 significantly correlated with the PASI. score (r=. 0.46, P<. 0.05; r=. 0.55, P<. 0.02, respectively). Receiver-operating characteristic analysis (ROC) showed that miR-223 and -143 have the potential to distinguish between psoriasis and healthy controls (miR-223: area under the curve (AUC). =. 0.80, miR-143: AUC. =. 0.75). Interestingly, after 3-5 weeks of treatment with methotrexate following a significant decrease in psoriasis severity, miR-223 and miR-143 were significantly downregulated in the PBMCs from patients with psoriasis. Conclusion: We suggest that changes in the miR-223 and miR-143 expressions in PBMCs from patients with psoriasis may serve as novel biomarkers for disease activity in psoriasis; however, further investigations are warranted to clarify their specific roles.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Dermatological Science |
| Vol/bind | 75 |
| Udgave nummer | 2 |
| Sider (fra-til) | 133-139 |
| Antal sider | 7 |
| ISSN | 0923-1811 |
| DOI | |
| Status | Udgivet - aug. 2014 |