Microbial biotransformation of five pyrrolidinophenone-type psychoactive substances in wastewater and a wastewater isolated Pseudomonas putida strain

TY - JOUR

T1 - Microbial biotransformation of five pyrrolidinophenone-type psychoactive substances in wastewater and a wastewater isolated Pseudomonas putida strain

AU - Mardal, Marie

AU - Bischoff, Markus

AU - Ibáñez, María

AU - Ruffing, Ulla

AU - Hernández, Félix

AU - Meyer, Markus R

N1 - Copyright © 2017 John Wiley & Sons, Ltd.

PY - 2017/10

Y1 - 2017/10

N2 - Wastewater-based epidemiology (WBE) employs the analysis of wastewater to detect and quantify drug use and discharge within a community. In this work, transformation products (TP) by microbes in the environment were identified after incubations in wastewater and an isolated microbial strain. The microbial strain was isolated from an enrichment culture of wastewater supplemented with 3,4-methylenedioxy-pyrovalerone, and identified by matrix assisted laser desorption – time of flight mass spectrometry as Pseudomonas putida (P. putida). Five pyrrolidinophenone-type psychoactive substances (PPPS) were then incubated in wastewater and in P. putida tryptic soy broth (TSB) growth cultures. TPs were identified using liquid chromatography coupled to mass spectrometry techniques. All TPs observed in P. putida TSB growth cultures were also identified in wastewater incubations. The main TP for all PPPSs in P. putida TSB growth cultures, and two PPPSs incubated in wastewater, were the N-desalkyl-carboxy-TPs. The study showed P. putida TSB growth cultures used for identification of TPs in wastewater, represent parts of the microbial community. With data provided in this type of experiments more information will be available to select targets for monitoring drug use by WBE.

AB - Wastewater-based epidemiology (WBE) employs the analysis of wastewater to detect and quantify drug use and discharge within a community. In this work, transformation products (TP) by microbes in the environment were identified after incubations in wastewater and an isolated microbial strain. The microbial strain was isolated from an enrichment culture of wastewater supplemented with 3,4-methylenedioxy-pyrovalerone, and identified by matrix assisted laser desorption – time of flight mass spectrometry as Pseudomonas putida (P. putida). Five pyrrolidinophenone-type psychoactive substances (PPPS) were then incubated in wastewater and in P. putida tryptic soy broth (TSB) growth cultures. TPs were identified using liquid chromatography coupled to mass spectrometry techniques. All TPs observed in P. putida TSB growth cultures were also identified in wastewater incubations. The main TP for all PPPSs in P. putida TSB growth cultures, and two PPPSs incubated in wastewater, were the N-desalkyl-carboxy-TPs. The study showed P. putida TSB growth cultures used for identification of TPs in wastewater, represent parts of the microbial community. With data provided in this type of experiments more information will be available to select targets for monitoring drug use by WBE.

KW - Journal Article

U2 - 10.1002/dta.2165

DO - 10.1002/dta.2165

M3 - Journal article

C2 - 28092435

SN - 1942-7603

VL - 9

SP - 1522

EP - 1536

JO - Drug Testing and Analysis

JF - Drug Testing and Analysis

IS - 10

ER -