Method enabling gene expression studies of pathogens in a complex food matrix

TY - JOUR

T1 - Method enabling gene expression studies of pathogens in a complex food matrix

AU - Kjeldgaard, Jette

AU - Henriksen, Sidsel

AU - Cohn, Marianne Thorup

AU - Aabo, Søren

AU - Ingmer, Hanne

PY - 2011/12

Y1 - 2011/12

N2 - We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.

AB - We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.

U2 - 10.1128/AEM.05471-11

DO - 10.1128/AEM.05471-11

M3 - Journal article

SN - 0099-2240

VL - 77

SP - 8456

EP - 8458

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 23

ER -