Metabolically stable cellular adhesion to inert surfaces

TY - JOUR

T1 - Metabolically stable cellular adhesion to inert surfaces

AU - Meldal, Morten Peter

AU - Wu, Boqian

AU - Diness, Frederik

AU - Michael, Roice

AU - Hagel, Grith

PY - 2011/11/4

Y1 - 2011/11/4

N2 - The structure of d-amino acid hexapeptides that promote cellular adhesion was determined by screening d-amino acid hexapeptide libraries synthesized on otherwise inert beaded PEGA resin. These new adhesion molecules provide a completely stable cellular environment and facilitate the maintenance of a monolayer of cells on beads for extended periods. The presence of the peptides promotes spreading of the cells on the bead surface. Not surprisingly, the molecules contained a significant number of arginines and/or lysines. However, the exact structure of each peptide is quite important for the degree of adhesion observed, and a motif with three or four basic amino acids spaced within amino acids of intermediate polarity clearly prevailed, for example, k-l/r-h-r-i/v-r-a; this maintains a polar/hydrophobic balance.

AB - The structure of d-amino acid hexapeptides that promote cellular adhesion was determined by screening d-amino acid hexapeptide libraries synthesized on otherwise inert beaded PEGA resin. These new adhesion molecules provide a completely stable cellular environment and facilitate the maintenance of a monolayer of cells on beads for extended periods. The presence of the peptides promotes spreading of the cells on the bead surface. Not surprisingly, the molecules contained a significant number of arginines and/or lysines. However, the exact structure of each peptide is quite important for the degree of adhesion observed, and a motif with three or four basic amino acids spaced within amino acids of intermediate polarity clearly prevailed, for example, k-l/r-h-r-i/v-r-a; this maintains a polar/hydrophobic balance.

M3 - Journal article

SN - 1439-4227

VL - 12

SP - 2463

EP - 2470

JO - ChemBioChem

JF - ChemBioChem

ER -