Mechanisms of activation of muscle branched-chain alpha-keto acid dehydrogenase during exercise in man

TY - JOUR

T1 - Mechanisms of activation of muscle branched-chain alpha-keto acid dehydrogenase during exercise in man

AU - Van Hall, Gerrit

AU - MacLean, D A

AU - Saltin, B

AU - Wagenmakers, A J

N1 - Keywords: 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); Adult; Exercise; Glycogen; Humans; Ketone Oxidoreductases; Knee; Male; Multienzyme Complexes; Muscle, Skeletal

PY - 1996

Y1 - 1996

N2 - 1. Exercise leads to activation (dephosphorylation) of the branched-chain alpha-keto acid dehydrogenase (BCKADH). Here we investigate the effect of low pre-exercise muscle glycogen content and of branched-chain amino acid (BCAA) ingestion on the activity of BCKADH at rest and after 90 min of one-leg knee-extensor exercise at 65% maximal one-leg power output in five subjects. 2. Pre-exercise BCAA ingestion (308 mg BCAAs (kg body wt)-1) caused an increased muscle BCAA uptake, a higher intramuscular BCAA concentration and activation of BCKADH both at rest (9 +/- 1 versus 25 +/- 5% for the control and BCAA test, respectively) and after exercise (27 +/- 4 versus 54 +/- 7%). 3. At rest the percentage active BCKADH was not different, 6 +/- 2% versus 5 +/- 1%, in the normal and low glycogen content leg (392 +/- 21 and 147 +/- 34 mumol glycosyl units (g dry muscle)-1, respectively). The post-exercise BCKADH activity was higher in the low (46 +/- 2%) than in the normal glycogen content leg (26 +/- 2%). 4. It is concluded that: (1) the mechanism of activation by BCAA ingestion probably involves an increase of the muscle BCAA concentration; (2) BCKADH activation caused by exercise and BCAA ingestion are additive; (3) low pre-exercise muscle glycogen content augments the exercise-induced BCKADH activation without an increase in muscle BCAA concentration; and (4) the mechanism of BCKADH activation via BCAA ingestion and low muscle glycogen content are different.

AB - 1. Exercise leads to activation (dephosphorylation) of the branched-chain alpha-keto acid dehydrogenase (BCKADH). Here we investigate the effect of low pre-exercise muscle glycogen content and of branched-chain amino acid (BCAA) ingestion on the activity of BCKADH at rest and after 90 min of one-leg knee-extensor exercise at 65% maximal one-leg power output in five subjects. 2. Pre-exercise BCAA ingestion (308 mg BCAAs (kg body wt)-1) caused an increased muscle BCAA uptake, a higher intramuscular BCAA concentration and activation of BCKADH both at rest (9 +/- 1 versus 25 +/- 5% for the control and BCAA test, respectively) and after exercise (27 +/- 4 versus 54 +/- 7%). 3. At rest the percentage active BCKADH was not different, 6 +/- 2% versus 5 +/- 1%, in the normal and low glycogen content leg (392 +/- 21 and 147 +/- 34 mumol glycosyl units (g dry muscle)-1, respectively). The post-exercise BCKADH activity was higher in the low (46 +/- 2%) than in the normal glycogen content leg (26 +/- 2%). 4. It is concluded that: (1) the mechanism of activation by BCAA ingestion probably involves an increase of the muscle BCAA concentration; (2) BCKADH activation caused by exercise and BCAA ingestion are additive; (3) low pre-exercise muscle glycogen content augments the exercise-induced BCKADH activation without an increase in muscle BCAA concentration; and (4) the mechanism of BCKADH activation via BCAA ingestion and low muscle glycogen content are different.

M3 - Journal article

C2 - 8865084

SN - 0022-3751

VL - 494 ( Pt 3)

SP - 899

EP - 905

JO - The Journal of Physiology

JF - The Journal of Physiology

ER -