TY - JOUR
T1 - Mechanics and dynamics of triglyceride-phospholipid model membranes
T2 - Implications for cellular properties and function
AU - Pakkanen, Kirsi I.
AU - Duelund, Lars
AU - Qvortrup, Klaus
AU - Ipsen, John Hjorth
N1 - Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2011/8
Y1 - 2011/8
N2 - We demonstrate here that triolein alters the mechanical properties of phospholipid membranes and induces extraordinary conformational dynamics. Triolein containing membranes exhibit fluctuations up to size range of 100µm and with the help of these are e.g. able to squeeze through narrow passages between neighbouring structures. Triolein-phosphatidylcholine membranes were found to have bending rigidity significantly lower than that of corresponding pure phosphatidylcholine membrane. Moreover, the triolein containing membranes were found to be reluctant to fuse, which is in good accordance with larger lamellar distances observed in the TOPOPC membranes. These findings suggest repulsion between adjacent membranes. We provide a comprehensive discussion on the possible explanations for the observed mechanics and dynamics in the TOPOPC system and on their potential cellular implications.
AB - We demonstrate here that triolein alters the mechanical properties of phospholipid membranes and induces extraordinary conformational dynamics. Triolein containing membranes exhibit fluctuations up to size range of 100µm and with the help of these are e.g. able to squeeze through narrow passages between neighbouring structures. Triolein-phosphatidylcholine membranes were found to have bending rigidity significantly lower than that of corresponding pure phosphatidylcholine membrane. Moreover, the triolein containing membranes were found to be reluctant to fuse, which is in good accordance with larger lamellar distances observed in the TOPOPC membranes. These findings suggest repulsion between adjacent membranes. We provide a comprehensive discussion on the possible explanations for the observed mechanics and dynamics in the TOPOPC system and on their potential cellular implications.
KW - Calorimetry, Differential Scanning
KW - Cell Membrane
KW - Elasticity
KW - Fluorescence Resonance Energy Transfer
KW - Membrane Fluidity
KW - Membrane Fusion
KW - Membranes, Artificial
KW - Microscopy, Confocal
KW - Microscopy, Electron, Transmission
KW - Microscopy, Phase-Contrast
KW - Molecular Conformation
KW - Phosphatidylcholines
KW - Scattering, Small Angle
KW - Triolein
U2 - 10.1016/j.bbamem.2011.04.006
DO - 10.1016/j.bbamem.2011.04.006
M3 - Journal article
C2 - 21545789
SN - 0304-4165
VL - 1808
SP - 1947
EP - 1956
JO - B B A - General Subjects
JF - B B A - General Subjects
IS - 8
ER -