Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

L K Poulsen; M F Pedersen; H J Malling; I Søndergaard; B Weeke

Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

L K Poulsen, M F Pedersen, H J Malling, I Søndergaard, B Weeke

15 Citationer (Scopus)

Abstract

For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.

Originalsprog	Engelsk
Tidsskrift	Allergy
Vol/bind	44
Udgave nummer	3
Sider (fra-til)	173-80
Antal sider	8
ISSN	0105-4538
Status	Udgivet - apr. 1989

Citationsformater

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title = "Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids",

abstract = "For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ({"}Maxisorp RAST{"}). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.",

keywords = "Allergens, Antigens, Fungal, Cladosporium, Epitopes, Humans, Immunoglobulin E, Radioallergosorbent Test, Journal Article, Research Support, Non-U.S. Gov't",

author = "Poulsen, {L K} and Pedersen, {M F} and Malling, {H J} and I S{\o}ndergaard and B Weeke",

year = "1989",

month = apr,

language = "English",

volume = "44",

pages = "173--80",

journal = "Allergy: European Journal of Allergy and Clinical Immunology",

issn = "0105-4538",

publisher = "Wiley Online",

number = "3",

}

TY - JOUR

T1 - Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

AU - Poulsen, L K

AU - Pedersen, M F

AU - Malling, H J

AU - Søndergaard, I

AU - Weeke, B

PY - 1989/4

Y1 - 1989/4

N2 - For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.

AB - For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.

KW - Allergens

KW - Antigens, Fungal

KW - Cladosporium

KW - Epitopes

KW - Humans

KW - Immunoglobulin E

KW - Radioallergosorbent Test

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 2469345

SN - 0105-4538

VL - 44

SP - 173

EP - 180

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

IS - 3

ER -

Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

Abstract

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