TY - JOUR
T1 - Matching NLR immune receptors to autoimmunity in camta3 mutants using antimorphic NLR alleles
AU - Lolle, Signe
AU - Greeff, Michael Christiaan
AU - Petersen, Klaus
AU - Roux, Milena Edna
AU - Jensen, Michael Krogh
AU - Bressendorff, Simon
AU - Rodriguez Gomes, Eleazar José
AU - Sømark, Kenneth
AU - Mundy, John
AU - Petersen, Morten
PY - 2017
Y1 - 2017
N2 - To establish infection, pathogens deploy effectors to modify or remove host proteins. Plant immune receptors with nucleotide-binding, leucine-rich repeat domains (NLRs) detect these modifications and trigger immunity. Plant NLRs thus guard host “guardees.” A corollary is that autoimmunity may result from inappropriate NLR activation because mutations in plant guardees could trigger corresponding NLR guards. To explore these hypotheses, we expressed 108 dominant-negative (DN) Arabidopsis NLRs in various lesion mimic mutants, including camta3, which exhibits autoimmunity. CAMTA3 was previously described as a negative regulator of immunity, and we find that autoimmunity in camta3 is fully suppressed by expressing DNs of two NLRs, DSC1 and DSC2. Additionally, expression of either NLR triggers cell death that can be suppressed by CAMTA3 expression. These findings support a model in which DSC1 and DSC2 guard CAMTA3, and they suggest that other negative regulators of immunity may similarly represent guardees.
AB - To establish infection, pathogens deploy effectors to modify or remove host proteins. Plant immune receptors with nucleotide-binding, leucine-rich repeat domains (NLRs) detect these modifications and trigger immunity. Plant NLRs thus guard host “guardees.” A corollary is that autoimmunity may result from inappropriate NLR activation because mutations in plant guardees could trigger corresponding NLR guards. To explore these hypotheses, we expressed 108 dominant-negative (DN) Arabidopsis NLRs in various lesion mimic mutants, including camta3, which exhibits autoimmunity. CAMTA3 was previously described as a negative regulator of immunity, and we find that autoimmunity in camta3 is fully suppressed by expressing DNs of two NLRs, DSC1 and DSC2. Additionally, expression of either NLR triggers cell death that can be suppressed by CAMTA3 expression. These findings support a model in which DSC1 and DSC2 guard CAMTA3, and they suggest that other negative regulators of immunity may similarly represent guardees.
UR - http://www.scopus.com/inward/record.url?scp=85017374778&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2017.03.005
DO - 10.1016/j.chom.2017.03.005
M3 - Journal article
C2 - 28407487
AN - SCOPUS:85017374778
SN - 1931-3128
VL - 21
SP - 518
EP - 529
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 4
ER -