Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats

Anders Jørgensen; Katrine Breitenstein; Otto Kalliokoski; Allan Weimann; Trine Henriksen; Henrik Enghusen Poulsen; Martin Balslev Jørgensen; Gitta Wörtwein

doi:10.1017/neu.2019.7

Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats

Anders Jørgensen^*, Katrine Breitenstein, Otto Kalliokoski, Allan Weimann, Trine Henriksen, Henrik Enghusen Poulsen, Martin Balslev Jørgensen, Gitta Wörtwein

^*Corresponding author af dette arbejde

1 Citationer (Scopus)

Abstract

Objective: Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT), a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side effects of ECT.Methods: We measured sensitive markers of systemic and central nervous system (CNS) oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS), an animal model of ECT. Due to the previous observations that link hypothalamic-pituitary-adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results: ECS induced weight loss, increased corticosterone (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion: These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young nor middle-aged rats.

Originalsprog	Engelsk
Tidsskrift	Acta Neuropsychiatrica
Vol/bind	31
Udgave nummer	6
Sider (fra-til)	287-293
Antal sider	7
ISSN	0924-2708
DOI	https://doi.org/10.1017/neu.2019.7
Status	Udgivet - 14 dec. 2019

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10.1017/neu.2019.7

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title = "Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats",

abstract = "Objective: Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT), a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side effects of ECT.Methods: We measured sensitive markers of systemic and central nervous system (CNS) oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS), an animal model of ECT. Due to the previous observations that link hypothalamic-pituitary-adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results: ECS induced weight loss, increased corticosterone (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion: These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young nor middle-aged rats.",

keywords = "DNA repair, Electroconvulsive stimulations, HPA-axis, nucleic acids, oxidative stress",

author = "Anders J{\o}rgensen and Katrine Breitenstein and Otto Kalliokoski and Allan Weimann and Trine Henriksen and Poulsen, {Henrik Enghusen} and J{\o}rgensen, {Martin Balslev} and Gitta W{\"o}rtwein",

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month = dec,

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doi = "10.1017/neu.2019.7",

language = "English",

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journal = "Acta Neuropsychiatrica",

issn = "0924-2708",

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TY - JOUR

T1 - Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats

AU - Jørgensen, Anders

AU - Breitenstein, Katrine

AU - Kalliokoski, Otto

AU - Weimann, Allan

AU - Henriksen, Trine

AU - Poulsen, Henrik Enghusen

AU - Jørgensen, Martin Balslev

AU - Wörtwein, Gitta

PY - 2019/12/14

Y1 - 2019/12/14

N2 - Objective: Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT), a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side effects of ECT.Methods: We measured sensitive markers of systemic and central nervous system (CNS) oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS), an animal model of ECT. Due to the previous observations that link hypothalamic-pituitary-adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results: ECS induced weight loss, increased corticosterone (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion: These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young nor middle-aged rats.

AB - Objective: Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT), a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side effects of ECT.Methods: We measured sensitive markers of systemic and central nervous system (CNS) oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS), an animal model of ECT. Due to the previous observations that link hypothalamic-pituitary-adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results: ECS induced weight loss, increased corticosterone (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion: These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young nor middle-aged rats.

KW - DNA repair

KW - Electroconvulsive stimulations

KW - HPA-axis

KW - nucleic acids

KW - oxidative stress

U2 - 10.1017/neu.2019.7

DO - 10.1017/neu.2019.7

M3 - Journal article

C2 - 30854991

AN - SCOPUS:85063165332

SN - 0924-2708

VL - 31

SP - 287

EP - 293

JO - Acta Neuropsychiatrica

JF - Acta Neuropsychiatrica

IS - 6

ER -

Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats

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