Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1

TY - JOUR

T1 - Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1

AU - Kusk, P

AU - Lindhardt, B O

AU - Bugge, T H

AU - Holmbäck, K

AU - Hulgaard, E F

PY - 1993/4

Y1 - 1993/4

N2 - A random fragment expression library was used to identify and map a new human epitope on the vpu protein of the human immunodeficiency virus type 1 (HIV-1). The epitope was mapped to the central part of the protein within amino acids (aa) 37-50 comprising the sequence N-KIDRLIDRLI-ERAE-C. A alpha-galactosidase-vpu fusion protein representing aa 37-68 of vpu was used to screen 356 human serum samples from HIV-1-infected persons for antibodies to the novel epitope. A total of 125 (35.1%) of the samples reacted with this region of vpu. Antibodies against this region were significantly more prevalent among samples from individuals with CD4 cell counts < 400 cells/microliters than individuals with CD4 cell counts > or = 400 cells/microliters (37.6 vs. 17.6%; p < 0.0146, Fisher's exact test). Thus, the presence of antibodies against this epitope of vpu appears to be associated with a progressed state of disease.

AB - A random fragment expression library was used to identify and map a new human epitope on the vpu protein of the human immunodeficiency virus type 1 (HIV-1). The epitope was mapped to the central part of the protein within amino acids (aa) 37-50 comprising the sequence N-KIDRLIDRLI-ERAE-C. A alpha-galactosidase-vpu fusion protein representing aa 37-68 of vpu was used to screen 356 human serum samples from HIV-1-infected persons for antibodies to the novel epitope. A total of 125 (35.1%) of the samples reacted with this region of vpu. Antibodies against this region were significantly more prevalent among samples from individuals with CD4 cell counts < 400 cells/microliters than individuals with CD4 cell counts > or = 400 cells/microliters (37.6 vs. 17.6%; p < 0.0146, Fisher's exact test). Thus, the presence of antibodies against this epitope of vpu appears to be associated with a progressed state of disease.

KW - Amino Acid Sequence

KW - CD4-Positive T-Lymphocytes

KW - Epitopes/analysis

KW - Gene Library

KW - HIV-1/genetics

KW - Human Immunodeficiency Virus Proteins

KW - Humans

KW - Leukocyte Count

KW - Male

KW - Molecular Sequence Data

KW - Viral Regulatory and Accessory Proteins/genetics

M3 - Journal article

C2 - 7681110

SN - 1525-4135

VL - 6

SP - 334

EP - 338

JO - J A I D S

JF - J A I D S

IS - 4

ER -