TY - JOUR
T1 - Liposome accumulation in irradiated tumors display important tumor and dose dependent differences
AU - Hansen, Anders Elias
AU - Fliedner, Frederikke Petrine
AU - Henriksen, Jonas Rosager
AU - Jørgensen, Jesper Tranekjær
AU - Clemmensen, Andreas Ettrup
AU - Børresen, Betina
AU - Elema, Dennis Ringkjøbing
AU - Kjær, Andreas
AU - Andresen, Thomas Lars
N1 - Copyright © 2017 Elsevier Inc. All rights reserved.
PY - 2018/1
Y1 - 2018/1
N2 - Radiation therapy may affect several important parameters in the tumor microenvironment and thereby influence the accumulation of liposomes by the enhanced permeability and retention (EPR)-effect. Here we investigate the effect of single dose radiation therapy on liposome tumor accumulation by PET/CT imaging using radiolabeled liposomes. Head and neck cancer xenografts (FaDu) and syngenic colorectal (CT26) cancer models were investigated. Radiotherapy displayed opposite effects in the two models. FaDu tumors displayed increased mean accumulation of liposomes for radiation doses up to 10 Gy, whereas CT26 tumors displayed a tendency for decreased accumulation. Tumor hypoxia was found negatively correlated to microregional distribution of liposomes. However, liposome distribution in relation to hypoxia was improved at lower radiation doses. The study reveals that the heterogeneity in liposome tumor accumulation between tumors and different radiation protocols are important factors that need to be taken into consideration to achieve optimal effect of liposome based radio-sensitizer therapy.
AB - Radiation therapy may affect several important parameters in the tumor microenvironment and thereby influence the accumulation of liposomes by the enhanced permeability and retention (EPR)-effect. Here we investigate the effect of single dose radiation therapy on liposome tumor accumulation by PET/CT imaging using radiolabeled liposomes. Head and neck cancer xenografts (FaDu) and syngenic colorectal (CT26) cancer models were investigated. Radiotherapy displayed opposite effects in the two models. FaDu tumors displayed increased mean accumulation of liposomes for radiation doses up to 10 Gy, whereas CT26 tumors displayed a tendency for decreased accumulation. Tumor hypoxia was found negatively correlated to microregional distribution of liposomes. However, liposome distribution in relation to hypoxia was improved at lower radiation doses. The study reveals that the heterogeneity in liposome tumor accumulation between tumors and different radiation protocols are important factors that need to be taken into consideration to achieve optimal effect of liposome based radio-sensitizer therapy.
KW - Animals
KW - Colorectal Neoplasms/diagnostic imaging
KW - Copper Radioisotopes/administration & dosage
KW - Female
KW - Gamma Rays/therapeutic use
KW - Head and Neck Neoplasms/diagnostic imaging
KW - Humans
KW - Hypoxia/metabolism
KW - Liposomes/administration & dosage
KW - Mice
KW - Mice, Nude
KW - Positron Emission Tomography Computed Tomography/methods
KW - Radiation Dosage
KW - Tissue Distribution
KW - Treatment Outcome
KW - Tumor Cells, Cultured
KW - Xenograft Model Antitumor Assays
U2 - 10.1016/j.nano.2017.08.013
DO - 10.1016/j.nano.2017.08.013
M3 - Journal article
C2 - 28882674
SN - 1549-9634
VL - 14
SP - 27
EP - 34
JO - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
IS - 1
ER -
