Lipid protrusions, membrane softness, and enzymatic activity

Pernille Høyrup; Thomas H. Callisen; Morten Jensen; Avi Halperin; Ole G. Mouritsen

doi:10.1039/b314146b

Lipid protrusions, membrane softness, and enzymatic activity

Pernille Høyrup, Thomas H. Callisen^*, Morten Jensen, Avi Halperin, Ole G. Mouritsen

^*Corresponding author af dette arbejde

Institut for Neurovidenskab

31 Citationer (Scopus)

Abstract

The activity of phospholipase A₂ on lipid bilayers displays a characteristic lag-burst behavior that has previously been shown to reflect the physical properties of the substrate. It has remained unclear which underlying molecular mechanism is responsible for this phenomenon. We propose here that protrusions of single lipid molecules out of the bilayer plane could provide such a mechanism. The proposal is supported by a combination of atomic-scale molecular dynamics simulations, theory, and experiments that have been performed in order to investigate the relationship between on the one side lipid protrusion modes and mechanical softness of phospholipid bilayers and on the other side the activity of enzymes acting on lipid bilayers composed of different unsaturated lipids. Specifically, our experiments show a correlation between the bilayer bending rigidity and the apparent Arrhenius activation energy extracted from systematic lag-time versus temperature analyses.

Originalsprog	Engelsk
Tidsskrift	Physical Chemistry Chemical Physics
Vol/bind	6
Udgave nummer	7
Sider (fra-til)	1608-1615
Antal sider	8
ISSN	1463-9076
DOI	https://doi.org/10.1039/b314146b
Status	Udgivet - 7 apr. 2004

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title = "Lipid protrusions, membrane softness, and enzymatic activity",

abstract = "The activity of phospholipase A2 on lipid bilayers displays a characteristic lag-burst behavior that has previously been shown to reflect the physical properties of the substrate. It has remained unclear which underlying molecular mechanism is responsible for this phenomenon. We propose here that protrusions of single lipid molecules out of the bilayer plane could provide such a mechanism. The proposal is supported by a combination of atomic-scale molecular dynamics simulations, theory, and experiments that have been performed in order to investigate the relationship between on the one side lipid protrusion modes and mechanical softness of phospholipid bilayers and on the other side the activity of enzymes acting on lipid bilayers composed of different unsaturated lipids. Specifically, our experiments show a correlation between the bilayer bending rigidity and the apparent Arrhenius activation energy extracted from systematic lag-time versus temperature analyses.",

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T1 - Lipid protrusions, membrane softness, and enzymatic activity

AU - Høyrup, Pernille

AU - Callisen, Thomas H.

AU - Jensen, Morten

AU - Halperin, Avi

AU - Mouritsen, Ole G.

PY - 2004/4/7

Y1 - 2004/4/7

N2 - The activity of phospholipase A2 on lipid bilayers displays a characteristic lag-burst behavior that has previously been shown to reflect the physical properties of the substrate. It has remained unclear which underlying molecular mechanism is responsible for this phenomenon. We propose here that protrusions of single lipid molecules out of the bilayer plane could provide such a mechanism. The proposal is supported by a combination of atomic-scale molecular dynamics simulations, theory, and experiments that have been performed in order to investigate the relationship between on the one side lipid protrusion modes and mechanical softness of phospholipid bilayers and on the other side the activity of enzymes acting on lipid bilayers composed of different unsaturated lipids. Specifically, our experiments show a correlation between the bilayer bending rigidity and the apparent Arrhenius activation energy extracted from systematic lag-time versus temperature analyses.

AB - The activity of phospholipase A2 on lipid bilayers displays a characteristic lag-burst behavior that has previously been shown to reflect the physical properties of the substrate. It has remained unclear which underlying molecular mechanism is responsible for this phenomenon. We propose here that protrusions of single lipid molecules out of the bilayer plane could provide such a mechanism. The proposal is supported by a combination of atomic-scale molecular dynamics simulations, theory, and experiments that have been performed in order to investigate the relationship between on the one side lipid protrusion modes and mechanical softness of phospholipid bilayers and on the other side the activity of enzymes acting on lipid bilayers composed of different unsaturated lipids. Specifically, our experiments show a correlation between the bilayer bending rigidity and the apparent Arrhenius activation energy extracted from systematic lag-time versus temperature analyses.

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U2 - 10.1039/b314146b

DO - 10.1039/b314146b

M3 - Journal article

AN - SCOPUS:2042508368

SN - 1463-9076

VL - 6

SP - 1608

EP - 1615

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 7

ER -

Lipid protrusions, membrane softness, and enzymatic activity

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