Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme

David Cannella; K. Benedikt Möllers; Niels-Ulrik Frigaard; Poul Erik Jensen; Morten Jannik Bjerrum; Katja Salomon Johansen; Claus Felby

doi:10.1038/ncomms11134

Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme

David Cannella, K. Benedikt Möllers, Niels-Ulrik Frigaard, Poul Erik Jensen, Morten Jannik Bjerrum, Katja Salomon Johansen, Claus Felby

102 Citationer (Scopus)

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Abstract

Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.

Originalsprog	Engelsk
Artikelnummer	11134
Tidsskrift	Nature Communications
Vol/bind	7
ISSN	2041-1723
DOI	https://doi.org/10.1038/ncomms11134
Status	Udgivet - 4 apr. 2016

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abstract = "Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.",

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AU - Cannella, David

AU - Möllers, K. Benedikt

AU - Frigaard, Niels-Ulrik

AU - Jensen, Poul Erik

AU - Bjerrum, Morten Jannik

AU - Johansen, Katja Salomon

AU - Felby, Claus

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N2 - Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.

AB - Oxidative processes are essential for the degradation of plant biomass. A class of powerful and widely distributed oxidative enzymes, the lytic polysaccharide monooxygenases (LPMOs), oxidize the most recalcitrant polysaccharides and require extracellular electron donors. Here we investigated the effect of using excited photosynthetic pigments as electron donors. LPMOs combined with pigments and reducing agents were exposed to light, which resulted in a never before seen 100-fold increase in catalytic activity. In addition, LPMO substrate specificity was broadened to include both cellulose and hemicellulose. LPMO enzymes and pigment derivatives common in the environment of plant-degrading organisms thus form a highly reactive and stable light-driven system increasing the turnover rate and versatility of LPMOs. This light-driven system may find applications in biotechnology and chemical processing.

U2 - 10.1038/ncomms11134

DO - 10.1038/ncomms11134

M3 - Journal article

C2 - 27041218

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 11134

ER -

Light-driven oxidation of polysaccharides by photosynthetic pigments and a metalloenzyme

Abstract

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