Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes

Weihuan Fang; Birgitte Bjørn Budde; Henrik Siegumfeldt

doi:10.1111/j.1574-6968.2006.00219.x

Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes

Weihuan Fang, Birgitte Bjørn Budde, Henrik Siegumfeldt

7 Citationer (Scopus)

Abstract

A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes, although the intracellular pH (pHi) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pHi occurred much more rapidly in liquid than in agar.

Originalsprog	Engelsk
Tidsskrift	FEMS Microbiology Letters
Vol/bind	258
Udgave nummer	2
Sider (fra-til)	208-213
Antal sider	6
ISSN	0378-1097
DOI	https://doi.org/10.1111/j.1574-6968.2006.00219.x
Status	Udgivet - 2006

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title = "Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes",

abstract = "A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes, although the intracellular pH (pHi) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pHi occurred much more rapidly in liquid than in agar.",

keywords = "Former LIFE faculty, Listeria monocytogenes; intracellular pH; Leuconostoc carnosum, leucocins 4010, bacteriocin",

author = "Weihuan Fang and Budde, {Birgitte Bj{\o}rn} and Henrik Siegumfeldt",

year = "2006",

doi = "10.1111/j.1574-6968.2006.00219.x",

language = "English",

volume = "258",

pages = "208--213",

journal = "F E M S Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "2",

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TY - JOUR

T1 - Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes

AU - Fang, Weihuan

AU - Budde, Birgitte Bjørn

AU - Siegumfeldt, Henrik

PY - 2006

Y1 - 2006

N2 - A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes, although the intracellular pH (pHi) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pHi occurred much more rapidly in liquid than in agar.

AB - A mixed culture of single cells of Listeria monocytogenes and the bacteriocin producing Leuconostoc carnosum 4010 showed growth inhibition of L. monocytogenes, although the intracellular pH (pHi) of L. monocytogenes followed by fluorescence ratio imaging microscopy was not affected. Furthermore, L. monocytogenes was exposed to the bacteriocins leucocins 4010 and nisin either in a liquid filled chamber or on the surface of an agar containing bacteriocins. Both bacteriocins caused dissipation of the pH gradient in L. monocytogenes and the effect was clearly dependent on the matrix, as the decrease in pHi occurred much more rapidly in liquid than in agar.

KW - Former LIFE faculty

KW - Listeria monocytogenes; intracellular pH; Leuconostoc carnosum, leucocins 4010, bacteriocin

U2 - 10.1111/j.1574-6968.2006.00219.x

DO - 10.1111/j.1574-6968.2006.00219.x

M3 - Journal article

C2 - 16640575

SN - 0378-1097

VL - 258

SP - 208

EP - 213

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

IS - 2

ER -

Leucocins 4010 from Leuconostoc carnosum cause a matrix related decrease in intracellular pH of Listeria monocytogenes

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