TY - JOUR
T1 - Leaf age-dependent effects of foliar-sprayed CuZn nanoparticles on photosynthetic efficiency and ROS generation in Arabidopsis thaliana
AU - Sperdouli, Ilektra
AU - Moustaka, Julietta
AU - Antonoglou, Orestis
AU - Adamakis, Ioannis Dimosthenis S.
AU - Dendrinou-Samara, Catherine
AU - Moustakas, Michael
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Young and mature leaves of Arabidopsis thaliana were exposed by foliar spray to 30 mg L-1 of CuZn nanoparticles (NPs). The NPs were synthesized by a microwave-assisted polyol process and characterized by dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). CuZn NPs effects in Arabidopsis leaves were evaluated by chlorophyll fluorescence imaging analysis that revealed spatiotemporal heterogeneity of the quantum efficiency of PSII photochemistry (ϕPSII) and the redox state of the plastoquinone (PQ) pool (qp), measured 30 min, 90 min, 180 min, and 240 min after spraying. Photosystem II (PSII) function in young leaves was observed to be negatively influenced, especially 30 min after spraying, at which point increased H2O2 generation was correlated to the lower oxidized state of the PQ pool. Recovery of young leaves photosynthetic efficiency appeared only after 240 min of NPs spray when also the level of ROS accumulation was similar to control leaves. On the contrary, a beneficial effect on PSII function in mature leaves after 30 min of the CuZn NPs spray was observed, with increased FPSII, an increased electron transport rate (ETR), decreased singlet oxygen (1O2) formation, and H2O2 production at the same level of control leaves.An explanation for this differential response is suggested.
AB - Young and mature leaves of Arabidopsis thaliana were exposed by foliar spray to 30 mg L-1 of CuZn nanoparticles (NPs). The NPs were synthesized by a microwave-assisted polyol process and characterized by dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). CuZn NPs effects in Arabidopsis leaves were evaluated by chlorophyll fluorescence imaging analysis that revealed spatiotemporal heterogeneity of the quantum efficiency of PSII photochemistry (ϕPSII) and the redox state of the plastoquinone (PQ) pool (qp), measured 30 min, 90 min, 180 min, and 240 min after spraying. Photosystem II (PSII) function in young leaves was observed to be negatively influenced, especially 30 min after spraying, at which point increased H2O2 generation was correlated to the lower oxidized state of the PQ pool. Recovery of young leaves photosynthetic efficiency appeared only after 240 min of NPs spray when also the level of ROS accumulation was similar to control leaves. On the contrary, a beneficial effect on PSII function in mature leaves after 30 min of the CuZn NPs spray was observed, with increased FPSII, an increased electron transport rate (ETR), decreased singlet oxygen (1O2) formation, and H2O2 production at the same level of control leaves.An explanation for this differential response is suggested.
KW - Bimetallic nanoparticles
KW - Hydrogen peroxide
KW - Mature leaves
KW - Non-photochemical quenching
KW - Photoprotective mechanism
KW - Photosynthetic heterogeneity
KW - Plastoquinone pool
KW - Redox state
KW - Spatiotemporal heterogeneity
KW - Young leaves
UR - http://www.scopus.com/inward/record.url?scp=85070614210&partnerID=8YFLogxK
U2 - 10.3390/ma12152498
DO - 10.3390/ma12152498
M3 - Journal article
C2 - 31390827
AN - SCOPUS:85070614210
SN - 1996-1944
VL - 12
SP - 1
EP - 17
JO - Materials
JF - Materials
IS - 15
M1 - 2498
ER -