Large-scale mRNA transfection of dendritic cells by electroporation in continuous flow systems

David Selmeczi; T. S. Hansen; Özcan Met; Inge Marie Svane; Niels B. Larsen

doi:10.1007/978-1-4939-3625-0_10

Large-scale mRNA transfection of dendritic cells by electroporation in continuous flow systems

David Selmeczi, T. S. Hansen, Özcan Met, Inge Marie Svane, Niels B. Larsen^*

^*Corresponding author af dette arbejde

Institut for Klinisk Medicin

1 Citationer (Scopus)

Abstract

Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 % with survival rates of >90 %. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.

Originalsprog	Engelsk
Titel	Methods in Molecular Biology
Antal sider	11
Vol/bind	1428
Forlag	Humana Press
Publikationsdato	2016
Sider	151-161
DOI	https://doi.org/10.1007/978-1-4939-3625-0_10
Status	Udgivet - 2016

Navn	Methods in Molecular Biology
Vol/bind	1428
ISSN	1064-3745

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10.1007/978-1-4939-3625-0_10

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abstract = "Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 % with survival rates of >90 %. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.",

keywords = "Dendritic cells, Electroporation, Laminar flow, Microfluidics, mRNA, Transfection",

author = "David Selmeczi and Hansen, {T. S.} and {\"O}zcan Met and Svane, {Inge Marie} and Larsen, {Niels B.}",

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T1 - Large-scale mRNA transfection of dendritic cells by electroporation in continuous flow systems

AU - Selmeczi, David

AU - Hansen, T. S.

AU - Met, Özcan

AU - Svane, Inge Marie

AU - Larsen, Niels B.

PY - 2016

Y1 - 2016

N2 - Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 % with survival rates of >90 %. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.

AB - Electroporation is well established for transient mRNA transfection of many mammalian cells, including immune cells such as dendritic cells used in cancer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synchronized electrical pulsing sequence can yield dendritic cell transfection rates of >75 % with survival rates of >90 %. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.

KW - Dendritic cells

KW - Electroporation

KW - Laminar flow

KW - Microfluidics

KW - mRNA

KW - Transfection

U2 - 10.1007/978-1-4939-3625-0_10

DO - 10.1007/978-1-4939-3625-0_10

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BT - Methods in Molecular Biology

PB - Humana Press

ER -

Large-scale mRNA transfection of dendritic cells by electroporation in continuous flow systems

Abstract

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