Large-scale extraction of rhamnogalacturonan I from industrial potato waste

TY - JOUR

T1 - Large-scale extraction of rhamnogalacturonan I from industrial potato waste

AU - Chrestensen, Inge Byg

AU - Diaz, Jerome Villarama

AU - Øgendal, Lars Holm

AU - Harholt, Jesper

AU - Jørgensen, Bodil

AU - Rolin, Claus

AU - Svava, Rikke

AU - Ulvskov, Peter

PY - 2012/4/15

Y1 - 2012/4/15

N2 - Potato pulp is rich in dietary fibres and is an underutilised material produced in large quantities by the potato starch factories. Potato fibres are especially rich in rhamnogalacturonan I (RG I). RG I is a pectic polysaccharide with a high degree of branching and until now undegraded RG I has only been extracted in small amounts limiting the application possibilities for RG I. The present paper describes a large-scale extraction process providing large quantities of undegraded RG I readily available. The extraction process includes enzymatic starch removal using purified Termamyl, enzymatic RG I solubilisation using a highly purified polygalacturonase, and finally purification using depth filtration and ultrafiltration. The extracted RG I has a high molecular weight and a monosaccharide composition comparable to RG I extracted by analytical extraction procedures. The large amount of RG I available by the presented method allows for thorough structure-function analyses and tailoring of RG I to specific functionalities.

AB - Potato pulp is rich in dietary fibres and is an underutilised material produced in large quantities by the potato starch factories. Potato fibres are especially rich in rhamnogalacturonan I (RG I). RG I is a pectic polysaccharide with a high degree of branching and until now undegraded RG I has only been extracted in small amounts limiting the application possibilities for RG I. The present paper describes a large-scale extraction process providing large quantities of undegraded RG I readily available. The extraction process includes enzymatic starch removal using purified Termamyl, enzymatic RG I solubilisation using a highly purified polygalacturonase, and finally purification using depth filtration and ultrafiltration. The extracted RG I has a high molecular weight and a monosaccharide composition comparable to RG I extracted by analytical extraction procedures. The large amount of RG I available by the presented method allows for thorough structure-function analyses and tailoring of RG I to specific functionalities.

U2 - 10.1016/j.foodchem.2011.09.106

DO - 10.1016/j.foodchem.2011.09.106

M3 - Journal article

SN - 0308-8146

VL - 131

SP - 1207

EP - 1216

JO - Food Chemistry

JF - Food Chemistry

IS - 4

ER -