Label-Free Shotgun Proteomics Approach to Characterize Muscle Tissue from Farmed and Wild European Sea Bass (Dicentrarchus labrax)

TY - JOUR

T1 - Label-Free Shotgun Proteomics Approach to Characterize Muscle Tissue from Farmed and Wild European Sea Bass (Dicentrarchus labrax)

AU - Chiozzi, Riccardo Zenezini

AU - Capriotti, Anna Laura

AU - Cavaliere, Chiara

AU - La Barbera, Giorgia

AU - Montone, Carmela Maria

AU - Piovesana, Susy

AU - Laganà, Aldo

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Sea bass represents one of the main fish products in the market. Most of it comes from farming and is bred in different conditons with respect to the wild fish. Differences may thus be expected. In this study, a proteomic profile of farmed and wild sea bass samples was performed, employing a fractionation strategy where peptide samples were first separated by 2D chromatography. The peptides were finally analyzed by shotgun proteomics workflow combined to tandem MS. The chosen fractionation approach was successful allowing to greatly improve the fish muscle protein characterization and detect some interesting differences between wild fish and farmed sea bass. Sixty-nine proteins were overexpressed in farmed fish samples, whereas 182 proteins were underexpressed. Some of these proteins could be related to the breeding conditions and the diet with which fishes were fed, thus providing some interesting results for assessing food quality based on a comprehensive proteomic study.

AB - Sea bass represents one of the main fish products in the market. Most of it comes from farming and is bred in different conditons with respect to the wild fish. Differences may thus be expected. In this study, a proteomic profile of farmed and wild sea bass samples was performed, employing a fractionation strategy where peptide samples were first separated by 2D chromatography. The peptides were finally analyzed by shotgun proteomics workflow combined to tandem MS. The chosen fractionation approach was successful allowing to greatly improve the fish muscle protein characterization and detect some interesting differences between wild fish and farmed sea bass. Sixty-nine proteins were overexpressed in farmed fish samples, whereas 182 proteins were underexpressed. Some of these proteins could be related to the breeding conditions and the diet with which fishes were fed, thus providing some interesting results for assessing food quality based on a comprehensive proteomic study.

KW - Bidimensional chromatography

KW - European sea bass

KW - Label free quantification

KW - Muscle tissue protein

KW - Shotgun proteomics

UR - http://www.scopus.com/inward/record.url?scp=85025479616&partnerID=8YFLogxK

U2 - 10.1007/s12161-017-0999-7

DO - 10.1007/s12161-017-0999-7

M3 - Journal article

AN - SCOPUS:85025479616

SN - 1936-9751

VL - 11

SP - 292

EP - 301

JO - Food Analytical Methods

JF - Food Analytical Methods

IS - 1

ER -