@article{910ae1f0e16c11ddb5fc000ea68e967b,
title = "Knocking out IL-6 by vaccination",
abstract = "Inappropriate expression of IL-6 plays a role in various inflammatory conditions, degenerative diseases, and cancers. Several model systems have been developed that can specifically block IL-6-receptor interactions. Here we present a simple and highly effective approach based on vaccination with a pool of specifically mutated IL-6 analogues to induce a neutralizing IL-6 antibody response in mice. Judged by the ability of the analogues to bind to heterologous anti-IL-6 antibodies and cellular IL-6 receptors the IL-6 analogues seemed to have a three-dimensional structure comparable to that of wild-type IL-6. Injection of them broke self-tolerance and induced an immune response to IL-6, presumably because of the amino acid differences between the analogues and wild-type IL-6. This resulted in a long-lasting anti-IL-6 antibody-mediated IL-6 deficiency that blocked experimentally induced IL-6-mediated pathology.",
author = "Pia Galle and Lotte Hougs and Torben Barington and Morten Svenson and Arne Svejgaard and Thomsen, {Allan Randrup} and Klaus Bendtzen and Hansen, {Morten B}",
note = "Keywords: Amino Acid Sequence; Animals; Antibodies; Female; Humans; Interleukin-6; Mice; Molecular Sequence Data; Protein Structure, Tertiary; Vaccines",
year = "2004",
doi = "10.1002/eji.200324338",
language = "English",
volume = "34",
pages = "291--300",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "1",
}
