TY - JOUR
T1 - KIT(D816V) Induces SRC-Mediated Tyrosine Phosphorylation of MITF and Altered Transcription Program in Melanoma
AU - Phung, Bengt
AU - Kazi, Julhash U
AU - Lundby, Alicia
AU - Bergsteinsdottir, Kristin
AU - Sun, Jianmin
AU - Goding, Colin R
AU - Jönsson, Göran
AU - Olsen, Jesper V
AU - Steingrímsson, Eiríkur
AU - Rönnstrand, Lars
N1 - ©2017 American Association for Cancer Research.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - The oncogenic D816V mutation of the KIT receptor is well characterized in systemic mastocytosis and acute myeloid leukemia. Although KITD816V has been found in melanoma, its function and involvement in this malignancy is not understood. Here we show that KITD816V induces tyrosine phosphorylation of MITF through a triple protein complex formation between KIT, MITF, and SRC family kinases. In turn, phosphorylated MITF activates target genes that are involved in melanoma proliferation, cellcycle progression, suppression of senescence, survival, and invasion. By blocking the triple protein complex formation, thus preventing MITF phosphorylation, the cells became hypersensitive to SRC inhibitors. We have therefore delineated a mechanism behind the oncogenic effects of KITD816V in melanoma and provided a rationale for the heightened SRC inhibitor sensitivity in KITD816V transformed cells. Implications: This study demonstrates that an oncogenic tyrosine kinase mutant, KITD816V, can alter the transcriptional program of the transcription factor MITF in melanoma.
AB - The oncogenic D816V mutation of the KIT receptor is well characterized in systemic mastocytosis and acute myeloid leukemia. Although KITD816V has been found in melanoma, its function and involvement in this malignancy is not understood. Here we show that KITD816V induces tyrosine phosphorylation of MITF through a triple protein complex formation between KIT, MITF, and SRC family kinases. In turn, phosphorylated MITF activates target genes that are involved in melanoma proliferation, cellcycle progression, suppression of senescence, survival, and invasion. By blocking the triple protein complex formation, thus preventing MITF phosphorylation, the cells became hypersensitive to SRC inhibitors. We have therefore delineated a mechanism behind the oncogenic effects of KITD816V in melanoma and provided a rationale for the heightened SRC inhibitor sensitivity in KITD816V transformed cells. Implications: This study demonstrates that an oncogenic tyrosine kinase mutant, KITD816V, can alter the transcriptional program of the transcription factor MITF in melanoma.
U2 - 10.1158/1541-7786.mcr-17-0149
DO - 10.1158/1541-7786.mcr-17-0149
M3 - Journal article
C2 - 28584020
SN - 1541-7786
VL - 15
SP - 1265
EP - 1274
JO - Molecular Cancer Research
JF - Molecular Cancer Research
IS - 9
ER -
