Kinetic competition between RNA Polymerase II and Sen1-dependent transcription termination

TY - JOUR

T1 - Kinetic competition between RNA Polymerase II and Sen1-dependent transcription termination

AU - Hazelbaker, Dane Z

AU - Marquardt, Sebastian

AU - Wlotzka, Wiebke

AU - Buratowski, Stephen

N1 - Copyright © 2013 Elsevier Inc. All rights reserved.

PY - 2013/1/10

Y1 - 2013/1/10

N2 - The essential helicase-like protein Sen1 mediates termination of RNA Polymerase II (Pol II) transcription at snoRNAs and other noncoding RNAs in yeast. A mutation in the Pol II subunit Rpb1 that increases the elongation rate increases read-through transcription at Sen1-mediated terminators. Termination and growth defects in sen1 mutant cells are partially suppressed by a slowly transcribing Pol II mutant and are exacerbated by a faster-transcribing Pol II mutant. Deletion of the nuclear exosome subunit Rrp6 allows visualization of noncoding RNA intermediates that are terminated but not yet processed. Sen1 mutants or faster-transcribing Pol II increase the average lengths of preprocessed snoRNA, CUT, and SUT transcripts, while slowed Pol II transcription produces shorter transcripts. These connections between transcription rate and Sen1 activity support a model whereby kinetic competition between elongating Pol II and Sen1 helicase establishes the temporal and spatial window for early Pol II termination.

AB - The essential helicase-like protein Sen1 mediates termination of RNA Polymerase II (Pol II) transcription at snoRNAs and other noncoding RNAs in yeast. A mutation in the Pol II subunit Rpb1 that increases the elongation rate increases read-through transcription at Sen1-mediated terminators. Termination and growth defects in sen1 mutant cells are partially suppressed by a slowly transcribing Pol II mutant and are exacerbated by a faster-transcribing Pol II mutant. Deletion of the nuclear exosome subunit Rrp6 allows visualization of noncoding RNA intermediates that are terminated but not yet processed. Sen1 mutants or faster-transcribing Pol II increase the average lengths of preprocessed snoRNA, CUT, and SUT transcripts, while slowed Pol II transcription produces shorter transcripts. These connections between transcription rate and Sen1 activity support a model whereby kinetic competition between elongating Pol II and Sen1 helicase establishes the temporal and spatial window for early Pol II termination.

KW - Alleles

KW - Amino Acid Motifs

KW - Amino Acid Substitution

KW - Chromosome Mapping

KW - DNA Helicases

KW - Kinetics

KW - RNA Helicases

KW - RNA Polymerase II

KW - RNA, Fungal

KW - RNA, Small Nucleolar

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Transcription Termination, Genetic

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.molcel.2012.10.014

DO - 10.1016/j.molcel.2012.10.014

M3 - Journal article

C2 - 23177741

SN - 1097-2765

VL - 49

SP - 55

EP - 66

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -