TY - JOUR
T1 - Keeping the intracellular vitamin C at a physiologically relevant level in endothelial cell culture
AU - Frikke-Schmidt, Henriette Rønne
AU - Lykkesfeldt, Jens
PY - 2010/2/1
Y1 - 2010/2/1
N2 - It is generally accepted that the addition of vitamin C to cell culture medium improves cell growth. However, once added, the vitamin C concentration declines rapidly. This situation differs from the in vivo environment where the endothelium is constantly supplied with ascorbate from the blood. With a focus on intracellular vitamin C, we simulated constant supply of ascorbate by the hourly addition of freshly prepared medium containing 75 μM ascorbate and subsequently compared it with more practical regimens using combinations of ascorbate and 2-phosphoascorbate. We found that a single supplement of ascorbate and 2-phosphoascorbate adequately maintains intracellular vitamin C at physiological levels for up to 72 h.
AB - It is generally accepted that the addition of vitamin C to cell culture medium improves cell growth. However, once added, the vitamin C concentration declines rapidly. This situation differs from the in vivo environment where the endothelium is constantly supplied with ascorbate from the blood. With a focus on intracellular vitamin C, we simulated constant supply of ascorbate by the hourly addition of freshly prepared medium containing 75 μM ascorbate and subsequently compared it with more practical regimens using combinations of ascorbate and 2-phosphoascorbate. We found that a single supplement of ascorbate and 2-phosphoascorbate adequately maintains intracellular vitamin C at physiological levels for up to 72 h.
U2 - 10.1016/j.ab.2009.09.040
DO - 10.1016/j.ab.2009.09.040
M3 - Journal article
C2 - 19782654
SN - 0003-2697
VL - 397
SP - 135
EP - 137
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -