Isolation of less than Glu-Ser-Leu-Arg-Trp-NH2, a novel neuropeptide from sea anemones

TY - JOUR

T1 - Isolation of less than Glu-Ser-Leu-Arg-Trp-NH2, a novel neuropeptide from sea anemones

AU - Ebbesen, Ditte Graff

AU - Grimmelikhuijzen, C J

PY - 1988/3/1

Y1 - 1988/3/1

N2 - Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2, a peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Ser-Leu-Arg-Trp-NH2. Using antisera to its carboxyterminal sequence Arg-Trp-NH2, the peptide was found to be exclusively localized in neurons of sea anemones, among them neurons associated with the sphincter muscle. This suggest that the peptide is a transmitter at neuromuscular junctions.

AB - Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2, a peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Ser-Leu-Arg-Trp-NH2. Using antisera to its carboxyterminal sequence Arg-Trp-NH2, the peptide was found to be exclusively localized in neurons of sea anemones, among them neurons associated with the sphincter muscle. This suggest that the peptide is a transmitter at neuromuscular junctions.

KW - Animals

KW - Chromatography, High Pressure Liquid

KW - Cnidaria

KW - Neurons

KW - Neuropeptides

KW - Oligopeptides

KW - Pyrrolidonecarboxylic Acid

KW - Sea Anemones

M3 - Journal article

C2 - 2897223

SN - 0006-8993

VL - 442

SP - 354

EP - 358

JO - Brain Research

JF - Brain Research

IS - 2

ER -