Isolation of high-affinity human IgE and IgG antibodies recognising Bet v 1 and Humicola lanuginosa lipase from combinatorial phage libraries

TY - JOUR

T1 - Isolation of high-affinity human IgE and IgG antibodies recognising Bet v 1 and Humicola lanuginosa lipase from combinatorial phage libraries

AU - Jakobsen, Charlotte G

AU - Bodtger, Uffe

AU - Kristensen, Peter

AU - Poulsen, Lars K.

AU - Roggen, Erwin L

PY - 2004/8

Y1 - 2004/8

N2 - Allergen-specific Fab fragments isolated from combinatorial IgE and IgG libraries are useful tools for studying allergen-antibody interactions. To characterise the interaction between different allergens and antibodies we have created recombinant human phage antibody libraries in the Fab format. Human IgE and IgG libraries have been created from patients allergic to birch pollen or lipase. These libraries have been used to select binders recognising the major birch pollen allergen Bet v 1 and Humicola lanuginosa lipase. A panel of allergen-specific IgE and IgG antibodies were identified; these were further characterised by allergen binding studies using Biacore and competition studies using human sera and antibodies purified from human sera. Affinities in the nM range were recorded and a competition with human sera for allergen binding was observed.

AB - Allergen-specific Fab fragments isolated from combinatorial IgE and IgG libraries are useful tools for studying allergen-antibody interactions. To characterise the interaction between different allergens and antibodies we have created recombinant human phage antibody libraries in the Fab format. Human IgE and IgG libraries have been created from patients allergic to birch pollen or lipase. These libraries have been used to select binders recognising the major birch pollen allergen Bet v 1 and Humicola lanuginosa lipase. A panel of allergen-specific IgE and IgG antibodies were identified; these were further characterised by allergen binding studies using Biacore and competition studies using human sera and antibodies purified from human sera. Affinities in the nM range were recorded and a competition with human sera for allergen binding was observed.

KW - Allergens

KW - Amino Acid Sequence

KW - Antigens, Plant

KW - B-Lymphocytes

KW - Base Sequence

KW - Humans

KW - Immunoglobulin E

KW - Immunoglobulin G

KW - Lipase

KW - Molecular Sequence Data

KW - Peptide Library

U2 - 10.1016/j.molimm.2004.05.009

DO - 10.1016/j.molimm.2004.05.009

M3 - Journal article

C2 - 15302157

SN - 0161-5890

VL - 41

SP - 941

EP - 953

JO - Molecular Immunology

JF - Molecular Immunology

IS - 10

ER -