Isolation and structure elucidation of cyclopeptide alkaloids from Ziziphus nummularia and Ziziphus spina-christi by HPLC-DAD-MS and HPLC-PDA-HRMS-SPE-NMR

TY - JOUR

T1 - Isolation and structure elucidation of cyclopeptide alkaloids from Ziziphus nummularia and Ziziphus spina-christi by HPLC-DAD-MS and HPLC-PDA-HRMS-SPE-NMR

AU - Tuenter, Emmy

AU - Foubert, Kenn

AU - Stærk, Dan

AU - Apers, Sandra

AU - Pieters, Luc

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Seven cyclopeptide alkaloids were isolated from the stem bark of Ziziphus nummularia and Ziziphus spina-christi. Three previously undescribed compounds were identified: nummularine-U, spinanine-B and spinanine-C, together with the known compounds mauritine-F, nummularine-D, nummularine-E and amphibine-D. For their purification either semi-preparative HPLC with DAD and ESIMS detection or HPLC-PDA-(HRMS)-SPE-NMR was applied, together with conventional separation methods. Their structures were elucidated by spectroscopic means.

AB - Seven cyclopeptide alkaloids were isolated from the stem bark of Ziziphus nummularia and Ziziphus spina-christi. Three previously undescribed compounds were identified: nummularine-U, spinanine-B and spinanine-C, together with the known compounds mauritine-F, nummularine-D, nummularine-E and amphibine-D. For their purification either semi-preparative HPLC with DAD and ESIMS detection or HPLC-PDA-(HRMS)-SPE-NMR was applied, together with conventional separation methods. Their structures were elucidated by spectroscopic means.

U2 - 10.1016/j.phytochem.2017.02.029

DO - 10.1016/j.phytochem.2017.02.029

M3 - Journal article

C2 - 28284565

SN - 0031-9422

VL - 138

SP - 163

EP - 169

JO - Phytochemistry

JF - Phytochemistry

ER -