Isolation and differentiation of stromal vascular cells to beige/brite cells

Ulrike Liisberg Aune; Lauren Ruiz; Shingo Kajimura

doi:10.3791/50191

Isolation and differentiation of stromal vascular cells to beige/brite cells

Ulrike Liisberg Aune, Lauren Ruiz, Shingo Kajimura

Cellebiologi og Fysiologi

88 Citationer (Scopus)

Abstract

Brown adipocytes have the ability to uncouple the respiratory chain in mitochondria and dissipate chemical energy as heat. Development of UCP1-positive brown adipocytes in white adipose tissues (so called beige or brite cells) is highly induced by a variety of environmental cues such as chronic cold exposure or by PPARγ agonists, therefore, this cell type has potential as a therapeutic target for obesity treatment. Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. The browning effect can be assessed by the expression of brown fat-selective markers such as UCP1.

Originalsprog	Engelsk
Artikelnummer	e50191
Tidsskrift	Journal of Visualized Experiments
Udgave nummer	73
ISSN	1940-087X
DOI	https://doi.org/10.3791/50191
Status	Udgivet - 2013

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title = "Isolation and differentiation of stromal vascular cells to beige/brite cells",

abstract = "Brown adipocytes have the ability to uncouple the respiratory chain in mitochondria and dissipate chemical energy as heat. Development of UCP1-positive brown adipocytes in white adipose tissues (so called beige or brite cells) is highly induced by a variety of environmental cues such as chronic cold exposure or by PPARγ agonists, therefore, this cell type has potential as a therapeutic target for obesity treatment. Although most immortalized adipocyte lines cannot recapitulate the process of {"}browning{"} of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. The browning effect can be assessed by the expression of brown fat-selective markers such as UCP1.",

keywords = "Adipocytes, Adipose tissue, Anatomy, Animal model, Beige/brite cells, Brown adipose tissue, Cell Differentiation, Cells, Cellular biology, Diabetes, Differentiation, Fat, Isolation, Issue 73, Medicine, Molecular biology, Obesity, Physiology, Primary adipocytes, Rosiglitazone, Stromal-vascular fraction, Surgery, Transcription factors, Uncoupling protein 1",

author = "Aune, {Ulrike Liisberg} and Lauren Ruiz and Shingo Kajimura",

year = "2013",

doi = "10.3791/50191",

language = "English",

journal = "Journal of Visualized Experiments",

issn = "1940-087X",

publisher = "Journal of Visualized Experiments",

number = "73",

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TY - JOUR

T1 - Isolation and differentiation of stromal vascular cells to beige/brite cells

AU - Aune, Ulrike Liisberg

AU - Ruiz, Lauren

AU - Kajimura, Shingo

PY - 2013

Y1 - 2013

N2 - Brown adipocytes have the ability to uncouple the respiratory chain in mitochondria and dissipate chemical energy as heat. Development of UCP1-positive brown adipocytes in white adipose tissues (so called beige or brite cells) is highly induced by a variety of environmental cues such as chronic cold exposure or by PPARγ agonists, therefore, this cell type has potential as a therapeutic target for obesity treatment. Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. The browning effect can be assessed by the expression of brown fat-selective markers such as UCP1.

AB - Brown adipocytes have the ability to uncouple the respiratory chain in mitochondria and dissipate chemical energy as heat. Development of UCP1-positive brown adipocytes in white adipose tissues (so called beige or brite cells) is highly induced by a variety of environmental cues such as chronic cold exposure or by PPARγ agonists, therefore, this cell type has potential as a therapeutic target for obesity treatment. Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. The browning effect can be assessed by the expression of brown fat-selective markers such as UCP1.

KW - Adipocytes

KW - Adipose tissue

KW - Anatomy

KW - Animal model

KW - Beige/brite cells

KW - Brown adipose tissue

KW - Cell Differentiation

KW - Cells

KW - Cellular biology

KW - Diabetes

KW - Differentiation

KW - Fat

KW - Isolation

KW - Issue 73

KW - Medicine

KW - Molecular biology

KW - Obesity

KW - Physiology

KW - Primary adipocytes

KW - Rosiglitazone

KW - Stromal-vascular fraction

KW - Surgery

KW - Transcription factors

KW - Uncoupling protein 1

U2 - 10.3791/50191

DO - 10.3791/50191

M3 - Journal article

C2 - 23568137

AN - SCOPUS:84875895887

SN - 1940-087X

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 73

M1 - e50191

ER -

Isolation and differentiation of stromal vascular cells to beige/brite cells

Abstract

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