Isolation and characterization of a soluble antigen complex of Plasmodium falciparum with pyrogenic properties

TY - JOUR

T1 - Isolation and characterization of a soluble antigen complex of Plasmodium falciparum with pyrogenic properties

AU - Jakobsen, P H

AU - Hviid, L

AU - Theander, T G

AU - Riley, E M

AU - Grellier, P

AU - Bruun, L S

AU - Dalsgaard, K

AU - Schrevel, J

AU - Jepsen, S

N1 - Keywords: Animals; Antigens, Protozoan; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Humans; Interleukin-1; Lymphocyte Activation; Plasmodium falciparum; Pyrogens; Rabbits; Solubility

PY - 1991

Y1 - 1991

N2 - A soluble antigen complex, previously designated antigen no. 7 (Ag7) on the basis of the pattern obtained by crossed immunoelectrophoresis of culture supernatants of P. falciparum, was isolated by affinity chromatography. It was shown to be synthesized at the schizont stage of the parasite growth cycle and to be located on the surface of the schizonts. Antibodies to Ag7 did not inhibit the growth of the parasite in vitro. Ag7 is recognized by immune human sera from many parts of the world and it stimulated the production of specific antibody in mice when incorporated into immune-stimulating complex (ISCOM) structures. It also specifically stimulated in vitro proliferation of lymphocytes from clinically immune adults. That it induced the secretion of interleukin 1 by human monocytes and was pyrogenic in rabbits was of particular interest. Thus Ag7 has endotoxin-like properties which make it a possible candidate for an antitoxic malaria vaccine.

AB - A soluble antigen complex, previously designated antigen no. 7 (Ag7) on the basis of the pattern obtained by crossed immunoelectrophoresis of culture supernatants of P. falciparum, was isolated by affinity chromatography. It was shown to be synthesized at the schizont stage of the parasite growth cycle and to be located on the surface of the schizonts. Antibodies to Ag7 did not inhibit the growth of the parasite in vitro. Ag7 is recognized by immune human sera from many parts of the world and it stimulated the production of specific antibody in mice when incorporated into immune-stimulating complex (ISCOM) structures. It also specifically stimulated in vitro proliferation of lymphocytes from clinically immune adults. That it induced the secretion of interleukin 1 by human monocytes and was pyrogenic in rabbits was of particular interest. Thus Ag7 has endotoxin-like properties which make it a possible candidate for an antitoxic malaria vaccine.

M3 - Journal article

C2 - 1993114

SN - 0903-4641

VL - 99

SP - 21

EP - 29

JO - Acta Pathologica Microbiologica et Immunologica Scandinavica

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica

IS - 1

ER -